Alterations in the metabolism of Pseudosuccinea columella (Mollusca: Gastropoda) caused by Heterorhabditis bacteriophora HP88 (Rhabditida: Heterorhabditidae)

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular and biochemical parasitology Pub Date : 2023-11-23 DOI:10.1016/j.molbiopara.2023.111599

Victor Menezes Tunholi , Natânia do Carmo Sperandio , Vinícius Menezes Tunholi-Alves , Lorena Souza Castro Altoé , Melissa Carvalho Machado do Couto-Chambarelli , Ludimila Santos Amaral , Caio Márcio de Oliveira Monteiro , Isabella Vilhena Freire Martins

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引用次数: 0

Abstract

The gastropod Pseudosuccinea columella participates in the dissemination of Fasciola hepatica in the environment, acting as the main intermediate host of this parasite in Brazil. The present study sought to elucidate the possible pathogenic effects of the entomopathogenic nematode (EPN) Heterorhabditis bacteriophora on P. columella, by evaluating the influence of infection on alanine aminotransferase (ALT) and aspartate aminotransferase (AST), as well as the concentrations of total protein, uric acid, and urea in the snail's hemolymph. For this, the snails were exposed to EPNs for 24 and 48 h, and for each exposure time, 20 snails were dissected after 7, 14 and 21 days for hemolymph collection. The primary findings suggest a significant proteolysis alongside elevated levels of uric acid and urea in P. columella infected individuals. These findings reveal that H. bacteriophora HP88 infection induced serious changes in the snail's metabolism, triggering important deleterious effects.

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由异habditis bacteriophora HP88 (rhabdida: heterorhabditida: Heterorhabditidae)引起的小柱伪琥珀藻(软体动物:腹足目)代谢的改变。

腹足动物Pseudosuccinea columella参与肝片形吸虫在环境中的传播，是巴西这种寄生虫的主要中间宿主。本研究旨在通过评估感染对蜗牛血淋巴中丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的影响，以及总蛋白、尿酸和尿素浓度的影响，阐明昆虫病原性线虫(EPN)异habditis bacteriophora对小柱藻的可能致病作用。为此，将钉螺暴露于EPNs 24和48小时，并在每个暴露时间分别于7、14和21天后解剖20只钉螺进行血淋巴收集。初步研究结果表明，在小柱假单胞菌感染个体中，尿酸和尿素水平升高，同时存在显著的蛋白质水解。这些发现表明，血吸虫HP88感染引起了蜗牛代谢的严重变化，引发了重要的有害影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.