Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population.

Journal of Forensic Medicine Pub Date : 2023-10-25 DOI:10.12116/j.issn.1004-5619.2022.520405

Zi-Wei Wang, Cheng-Tao Li, Xi-Ling Liu

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Abstract

Objectives: To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population, and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms.

Methods: According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population, the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing (NGS). After submitting DNA methylation levels to the age estimation model, the DNA methylation ages were predicted and compared with their real ages.

Results: The 6 DNA methylation sites in both detection techniques were age-related, with an R² of 0.85 and a median absolute deviation (MAD) of 4.81 years when using pyrosequencing；with an R² of 0.84 and MAD of 4.41 years when using NGS.

Conclusions: The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.

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DNA甲基化年龄估计模型在中国东部汉族人群中的跨平台应用

目的:评价基于DNA甲基化的中国东部汉族人群年龄估计模型的法医应用价值，为探索适合不同检测平台的年龄估计模型提供理论依据。方法:根据已发表的中国汉族人群血液DNA甲基化年龄估计模型中6个与年龄相关的甲基化位点，采用焦磷酸测序和下一代测序(NGS)检测48份样本的DNA甲基化水平。将DNA甲基化水平提交给年龄估计模型后，预测DNA甲基化年龄并将其与实际年龄进行比较。结果:两种检测技术的6个DNA甲基化位点均与年龄相关，使用焦磷酸测序时，R2为0.85，中位绝对偏差(MAD)为4.81年;使用NGS时，R2为0.84,MAD为4.41年。结论:基于NGS的焦磷酸测序和多用途区域甲基化富集测序技术下的血液DNA甲基化年龄估计模型能够准确估计年龄。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Forensic Medicine

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