Characterisation of the mating-type loci in species of Elsinoe causing scab diseases

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
N.Q. Pham , T.A. Duong , B.D. Wingfield , I. Barnes , A. Durán , M.J. Wingfield
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引用次数: 0

Abstract

The genus Elsinoe includes many aggressive plant pathogens that infect various economically important agricultural, horticultural and forestry plants. Significant diseases include citrus scab caused by E. fawcettii and E. australis, grapevine spot anthracnose by E. ampelina, and the emerging Eucalyptus scab and shoot malformation disease caused by the recently described E. necatrix. Despite their importance as plant pathogens, little is known regarding the biology of many Elsinoe spp. To gain insights into the reproductive biology of these fungi, we characterized the mating-type loci of seven species using whole genome sequence data. Results showed that the MAT1 locus organization and its flanking genes is relatively conserved in most cases. All seven species manifested a typical heterothallic mating system characterized by having either the MAT1-1 or MAT1-2 idiomorph present in an isolate. These idiomorphs were defined by the MAT1-1-1 or the MAT1-2-1 gene, respectively. A unique MAT1-1 idiomorph containing a truncated MAT1-2-1 gene, and a MAT1-1-1 gene, was identified in E. necatrix and E. fawcettii genomes. Additionally, two idiomorph-specific proteins were found in the MAT1-1 and MAT1-2 idiomorphs of E. australis. Universal mating-type markers confirmed heterothallism across 21 Elsinoe spp., are poised to advance future studies regarding the biology of these fungi.

引起结痂病的Elsinoe种交配型位点的特征
Elsinoe属包括许多侵袭性植物病原体,感染各种经济上重要的农业,园艺和林业植物。主要病害包括由弗西蒂E.和南方E.引起的柑橘痂病,由琥珀E.引起的葡萄斑疹炭疽病,以及最近描述的由necatrix引起的新出现的桉树痂病和芽部畸形病。尽管它们是重要的植物病原体,但对许多Elsinoe spp的生物学知之甚少。为了深入了解这些真菌的生殖生物学,我们使用全基因组序列数据对7种真菌的交配型位点进行了表征。结果表明,在大多数情况下,MAT1位点的组织结构及其侧翼基因相对保守。所有7个物种均表现出典型的异thallic交配系统,其特征是在分离物中存在MAT1-1或MAT1-2特异形态。这些自胚分别由MAT1-1-1或MAT1-2-1基因定义。在雌蜂和fawcettii基因组中发现了一个独特的MAT1-1二形态,其中包含一个截断的MAT1-2-1基因和一个MAT1-1-1基因。此外,在南棘的MAT1-1和MAT1-2自形中发现了两个自形特异性蛋白。普遍的交配型标记证实了21种Elsinoe属的异源性,这将为进一步研究这些真菌的生物学特性奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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