N.Q. Pham , T.A. Duong , B.D. Wingfield , I. Barnes , A. Durán , M.J. Wingfield
{"title":"Characterisation of the mating-type loci in species of Elsinoe causing scab diseases","authors":"N.Q. Pham , T.A. Duong , B.D. Wingfield , I. Barnes , A. Durán , M.J. Wingfield","doi":"10.1016/j.funbio.2023.11.003","DOIUrl":null,"url":null,"abstract":"<div><p>The genus <em>Elsinoe</em> includes many aggressive plant pathogens that infect various economically important agricultural, horticultural and forestry plants. Significant diseases include citrus scab caused by <em>E. fawcettii</em> and <em>E. australis</em>, grapevine spot anthracnose by <em>E. ampelina</em>, and the emerging Eucalyptus scab and shoot malformation disease caused by the recently described <em>E. necatrix</em>. Despite their importance as plant pathogens, little is known regarding the biology of many <em>Elsinoe spp</em>. To gain insights into the reproductive biology of these fungi, we characterized the mating-type loci of seven species using whole genome sequence data. Results showed that the <em>MAT1</em> locus organization and its flanking genes is relatively conserved in most cases. All seven species manifested a typical heterothallic mating system characterized by having either the <em>MAT1-1</em> or <em>MAT1-2</em> idiomorph present in an isolate. These idiomorphs were defined by the <em>MAT1-1-1</em> or the <em>MAT1-2-1</em> gene, respectively. A unique <em>MAT1-1</em> idiomorph containing a truncated <em>MAT1-2-1</em> gene, and a <em>MAT1-1-1</em> gene, was identified in <em>E. necatrix</em> and <em>E. fawcettii</em> genomes. Additionally, two idiomorph-specific proteins were found in the <em>MAT1-1</em> and <em>MAT1-2</em> idiomorphs of <em>E. australis</em>. Universal mating-type markers confirmed heterothallism across 21 <em>Elsinoe spp.</em>, are poised to advance future studies regarding the biology of these fungi.</p></div>","PeriodicalId":12683,"journal":{"name":"Fungal biology","volume":"127 12","pages":"Pages 1484-1490"},"PeriodicalIF":2.9000,"publicationDate":"2023-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1878614623001149/pdfft?md5=901c15ce3937d4b07c26db1316ca148c&pid=1-s2.0-S1878614623001149-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fungal biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1878614623001149","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The genus Elsinoe includes many aggressive plant pathogens that infect various economically important agricultural, horticultural and forestry plants. Significant diseases include citrus scab caused by E. fawcettii and E. australis, grapevine spot anthracnose by E. ampelina, and the emerging Eucalyptus scab and shoot malformation disease caused by the recently described E. necatrix. Despite their importance as plant pathogens, little is known regarding the biology of many Elsinoe spp. To gain insights into the reproductive biology of these fungi, we characterized the mating-type loci of seven species using whole genome sequence data. Results showed that the MAT1 locus organization and its flanking genes is relatively conserved in most cases. All seven species manifested a typical heterothallic mating system characterized by having either the MAT1-1 or MAT1-2 idiomorph present in an isolate. These idiomorphs were defined by the MAT1-1-1 or the MAT1-2-1 gene, respectively. A unique MAT1-1 idiomorph containing a truncated MAT1-2-1 gene, and a MAT1-1-1 gene, was identified in E. necatrix and E. fawcettii genomes. Additionally, two idiomorph-specific proteins were found in the MAT1-1 and MAT1-2 idiomorphs of E. australis. Universal mating-type markers confirmed heterothallism across 21 Elsinoe spp., are poised to advance future studies regarding the biology of these fungi.
期刊介绍:
Fungal Biology publishes original contributions in all fields of basic and applied research involving fungi and fungus-like organisms (including oomycetes and slime moulds). Areas of investigation include biodeterioration, biotechnology, cell and developmental biology, ecology, evolution, genetics, geomycology, medical mycology, mutualistic interactions (including lichens and mycorrhizas), physiology, plant pathology, secondary metabolites, and taxonomy and systematics. Submissions on experimental methods are also welcomed. Priority is given to contributions likely to be of interest to a wide international audience.