Three-dimensional morphometry of mammalian cells. I. Diameters.

C Ocqueteau, M Cury, L Becker, E Morgado, U González, L Muxica, B Gunther
{"title":"Three-dimensional morphometry of mammalian cells. I. Diameters.","authors":"C Ocqueteau,&nbsp;M Cury,&nbsp;L Becker,&nbsp;E Morgado,&nbsp;U González,&nbsp;L Muxica,&nbsp;B Gunther","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Three-dimensional measurements of eleven kinds of cells, obtained from serial sections of five different organs, excised from eleven adult mammals of different body sizes-from a 40 g mouse to a 450 kg cow-were made. In order to minimize technical errors all organs were submitted to standardized fixation and staining procedures. Twenty cell diameters (at the nuclear level) were measured in each of the 7 microns serial tissue section which were made in two planes, after a 90 degree rotation of the fixed and embedded organ specimens. The mean values of the cell diameter measurements were submitted to a cluster analysis by means of a computer program, to establish the cell type groups with similar morphometric characteristics. The dendrograms of the cell-type groupings were then compared with the results obtained by applying the traditional statistical analysis of the cell sizes (in micrometers) in the three dimensions of space, and also with the principal component analysis. With the three statistical methods we came to analogous conclusions. The empirical allometric exponents for the three cell diameters, when expressed independently as functions of body mass, are not significantly different from zero, and in consequence cell sizes are independent of body mass. The physiological meaning of the body-size-independence of the mean three cell diameters is discussed.</p>","PeriodicalId":75552,"journal":{"name":"Archivos de biologia y medicina experimentales","volume":"22 2","pages":"89-95"},"PeriodicalIF":0.0000,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archivos de biologia y medicina experimentales","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Three-dimensional measurements of eleven kinds of cells, obtained from serial sections of five different organs, excised from eleven adult mammals of different body sizes-from a 40 g mouse to a 450 kg cow-were made. In order to minimize technical errors all organs were submitted to standardized fixation and staining procedures. Twenty cell diameters (at the nuclear level) were measured in each of the 7 microns serial tissue section which were made in two planes, after a 90 degree rotation of the fixed and embedded organ specimens. The mean values of the cell diameter measurements were submitted to a cluster analysis by means of a computer program, to establish the cell type groups with similar morphometric characteristics. The dendrograms of the cell-type groupings were then compared with the results obtained by applying the traditional statistical analysis of the cell sizes (in micrometers) in the three dimensions of space, and also with the principal component analysis. With the three statistical methods we came to analogous conclusions. The empirical allometric exponents for the three cell diameters, when expressed independently as functions of body mass, are not significantly different from zero, and in consequence cell sizes are independent of body mass. The physiological meaning of the body-size-independence of the mean three cell diameters is discussed.

哺乳动物细胞的三维形态计量学。一、直径。
研究人员对11种细胞进行了三维测量,这些细胞是从11种不同体型的成年哺乳动物(从体重40克的老鼠到体重450公斤的奶牛)的5个不同器官的连续切片中获得的。为了尽量减少技术上的错误,所有器官都进行了标准化的固定和染色程序。在固定和嵌入器官标本90度旋转后,在两个平面上制作的7微米系列组织切片中,每个切片测量20个细胞直径(在核水平上)。细胞直径测量的平均值通过计算机程序进行聚类分析,建立具有相似形态特征的细胞类型组。然后将细胞类型分组的树突图与传统的三维空间细胞尺寸(微米)统计分析结果以及主成分分析结果进行比较。用这三种统计方法,我们得出了类似的结论。三种细胞直径的经验异速生长指数,当作为体重的函数独立表达时,与零没有显著差异,因此细胞大小与体重无关。讨论了三种细胞平均直径与体大小无关的生理意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信