Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.

IF 5.7 3区生物学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Biological Engineering Pub Date : 2023-11-23 DOI:10.1186/s13036-023-00393-1

Meike Bielfeldt, Kai Budde-Sagert, Nikolai Weis, Maren Buenning, Susanne Staehlke, Julius Zimmermann, Nils Arbeiter, Sahba Mobini, María Ujué González, Henrike Rebl, Adelinde Uhrmacher, Ursula van Rienen, Barbara Nebe

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引用次数: 0

Abstract

Background: Electrical stimulation is used for enhanced bone fracture healing. Electrochemical processes occur during the electrical stimulation at the electrodes and influence cellular reactions. Our approach aimed to distinguish between electrochemical and electric field effects on osteoblast-like MG-63 cells. We applied 20 Hz biphasic pulses via platinum electrodes for 2 h. The electrical stimulation of the cell culture medium and subsequent application to cells was compared to directly stimulated cells. The electric field distribution was predicted using a digital twin.

Results: Cyclic voltammetry and electrochemical impedance spectroscopy revealed partial electrolysis at the electrodes, which was confirmed by increased concentrations of hydrogen peroxide in the medium. While both direct stimulation and AC-conditioned medium decreased cell adhesion and spreading, only the direct stimulation enhanced the intracellular calcium ions and reactive oxygen species.

Conclusion: The electrochemical by-product hydrogen peroxide is not the main contributor to the cellular effects of electrical stimulation. However, undesired effects like decreased adhesion are mediated through electrochemical products in stimulated medium. Detailed characterisation and monitoring of the stimulation set up and electrochemical reactions are necessary to find safe electrical stimulation protocols.

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脉冲电刺激与电化学条件介质对人成骨细胞影响的区别。

背景:电刺激用于促进骨折愈合。电化学过程发生在电极的电刺激过程中，并影响细胞反应。我们的方法旨在区分电化学和电场对成骨细胞样MG-63细胞的影响。我们通过铂电极施加20 Hz双相脉冲2小时。将细胞培养基的电刺激和随后的细胞应用与直接刺激细胞进行比较。利用数字孪生预测了电场分布。结果:循环伏安法和电化学阻抗谱显示电极发生部分电解，过氧化氢浓度的增加证实了这一点。直接刺激和交流调节培养基均能降低细胞黏附和扩散，但只有直接刺激能增强细胞内钙离子和活性氧。结论:电化学副产物过氧化氢不是电刺激细胞效应的主要因素。然而，不希望的影响，如减少附着力是介导通过电化学产物在受激介质。为了找到安全的电刺激方案，对刺激装置和电化学反应进行详细的表征和监测是必要的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biological Engineering BIOCHEMICAL RESEARCH METHODS-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

7.10

自引率

1.80%

发文量

审稿时长

17 weeks

期刊介绍： Biological engineering is an emerging discipline that encompasses engineering theory and practice connected to and derived from the science of biology, just as mechanical engineering and electrical engineering are rooted in physics and chemical engineering in chemistry. Topical areas include, but are not limited to: Synthetic biology and cellular design Biomolecular, cellular and tissue engineering Bioproduction and metabolic engineering Biosensors Ecological and environmental engineering Biological engineering education and the biodesign process As the official journal of the Institute of Biological Engineering, Journal of Biological Engineering provides a home for the continuum from biological information science, molecules and cells, product formation, wastes and remediation, and educational advances in curriculum content and pedagogy at the undergraduate and graduate-levels. Manuscripts should explore commonalities with other fields of application by providing some discussion of the broader context of the work and how it connects to other areas within the field.