Comparative Analysis of miRNA and EMT Markers in Metastatic Colorectal Cancer.

IF 1.8 4区医学 Q3 ONCOLOGY

Cancer Investigation Pub Date : 2023-12-01 Epub Date: 2024-01-02 DOI:10.1080/07357907.2023.2283495

Veronika Hanusova, Petra Matouskova, Monika Manethova, Jiri Soukup, Stanislav John, Martin Zofka, Hana Vošmikova, Lukas Krbal, Emil Rudolf

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引用次数: 0

Abstract

Colorectal cancer (CRC) is the fourth most commonly diagnosed malignant condition in the world. Micro RNAs (miRNAs) as well as epithelial to mesenchymal transition (EMT) play an important role in the pathogenesis of CRC. We performed a comparative analysis of the expression of selected miRNA genes and EMT markers in bioptic samples from patients (n = 45) with primary CRC or metastatic (m)CRC to the regional lymph node using reverse transcription-quantitative PCR and IHC staining. Results: Out of all miRNA analyzed, the miR-17 expression was most significantly different and associated with lower risk of CRC spread to the lymph node. In addition, significant relationships were found between the tumor side localization and several miRNAs expressions (miR-9, miR-29b, miR-19a, miR-19b, miR-21, miR-106a, miR-20a and miR-17). In addition, of the examined EMT markers, only VEGFA expression correlated with tumor progression (tumor grade G2). In the examined set of patient samples and their matched healthy tissue, several specific molecular markers (miRNAs associated with EMT and tumor progression) were identified with a promising prognostic potential. Their further examination in larger patient cohorts is planned to validate the present data.

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转移性结直肠癌miRNA和EMT标志物的比较分析。

结直肠癌(CRC)是世界上第四大最常诊断的恶性疾病。微rna (miRNAs)和上皮到间充质转化(EMT)在结直肠癌的发病机制中起重要作用。我们使用逆转录定量PCR和免疫组化染色对原发性结直肠癌或转移性结直肠癌患者(n = 45)的活检样本中选定的miRNA基因和EMT标志物的表达进行了比较分析。结果:在所分析的所有miRNA中，miR-17的表达差异最为显著，并与结直肠癌扩散到淋巴结的风险较低相关。此外，肿瘤侧定位与几种mirna表达(miR-9、miR-29b、miR-19a、miR-19b、miR-21、miR-106a、miR-20a和miR-17)之间存在显著相关性。此外，在所检测的EMT标志物中，只有VEGFA表达与肿瘤进展相关(肿瘤分级G2)。在检查的患者样本及其匹配的健康组织中，几个特定的分子标记(与EMT和肿瘤进展相关的mirna)被确定为具有良好的预后潜力。他们计划在更大的患者队列中进行进一步的检查，以验证目前的数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cancer Investigation 医学-肿瘤学

CiteScore

3.80

自引率

4.20%

发文量

审稿时长

8.5 months

期刊介绍： Cancer Investigation is one of the most highly regarded and recognized journals in the field of basic and clinical oncology. It is designed to give physicians a comprehensive resource on the current state of progress in the cancer field as well as a broad background of reliable information necessary for effective decision making. In addition to presenting original papers of fundamental significance, it also publishes reviews, essays, specialized presentations of controversies, considerations of new technologies and their applications to specific laboratory problems, discussions of public issues, miniseries on major topics, new and experimental drugs and therapies, and an innovative letters to the editor section. One of the unique features of the journal is its departmentalized editorial sections reporting on more than 30 subject categories covering the broad spectrum of specialized areas that together comprise the field of oncology. Edited by leading physicians and research scientists, these sections make Cancer Investigation the prime resource for clinicians seeking to make sense of the sometimes-overwhelming amount of information available throughout the field. In addition to its peer-reviewed clinical research, the journal also features translational studies that bridge the gap between the laboratory and the clinic.