A novel monoclonal antibody against porcine macrophage colony-stimulating factor (CSF1) detects expression on the cell surface of macrophages

IF 1.4 3区农林科学 Q4 IMMUNOLOGY

Veterinary immunology and immunopathology Pub Date : 2023-11-18 DOI:10.1016/j.vetimm.2023.110681

Lindsey A. Waddell , Zhiguang Wu , Kristin A. Sauter , Jayne C. Hope , David A. Hume

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引用次数: 0

Abstract

Macrophage colony-stimulating factor (CSF1) controls the proliferation and differentiation of cells of the mononuclear phagocyte system through binding to the receptor CSF1R. The expression and function of CSF1 has been well-studied in rodents and humans, but knowledge is lacking in other veterinary species. The development of a novel mouse anti-porcine CSF1 monoclonal antibody (mAb) facilitates the characterisation of this growth factor in pigs. Cell surface expression of CSF1 was confirmed on differentiated macrophage populations derived from blood and bone marrow monocytes, and on lung resident macrophages, the first species for this to be confirmed. However, monocytes isolated from blood and bone marrow lacked CSF1 expression. This species-specific mAb delivers the opportunity to further understanding of porcine myeloid cell biology. This is not only vital for the role of pigs as a model for human health, but also as a veterinary species of significant economic and agricultural importance.

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一种新的抗猪巨噬细胞集落刺激因子(CSF1)单克隆抗体检测巨噬细胞表面的表达。

巨噬细胞集落刺激因子(Macrophage colony-stimulating factor, CSF1)通过与受体CSF1R结合，控制单核吞噬细胞系统细胞的增殖和分化。CSF1在啮齿动物和人类中的表达和功能已经得到了很好的研究，但在其他兽医物种中缺乏相关知识。一种新型小鼠抗猪CSF1单克隆抗体(mAb)的开发有助于猪体内该生长因子的表征。CSF1在来自血液和骨髓单核细胞的分化巨噬细胞群体和肺常驻巨噬细胞上的细胞表面表达得到证实，这是第一个被证实的物种。然而，从血液和骨髓中分离的单核细胞缺乏CSF1表达。这种物种特异性单抗提供了进一步了解猪髓细胞生物学的机会。这不仅对猪作为人类健康模范的作用至关重要，而且对作为具有重要经济和农业意义的兽医物种也至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary immunology and immunopathology 农林科学-免疫学

CiteScore

3.40

自引率

5.60%

发文量

审稿时长

70 days

期刊介绍： The journal reports basic, comparative and clinical immunology as they pertain to the animal species designated here: livestock, poultry, and fish species that are major food animals and companion animals such as cats, dogs, horses and camels, and wildlife species that act as reservoirs for food, companion or human infectious diseases, or as models for human disease. Rodent models of infectious diseases that are of importance in the animal species indicated above,when the disease requires a level of containment that is not readily available for larger animal experimentation (ABSL3), will be considered. Papers on rabbits, lizards, guinea pigs, badgers, armadillos, elephants, antelope, and buffalo will be reviewed if the research advances our fundamental understanding of immunology, or if they act as a reservoir of infectious disease for the primary animal species designated above, or for humans. Manuscripts employing other species will be reviewed if justified as fitting into the categories above. The following topics are appropriate: biology of cells and mechanisms of the immune system, immunochemistry, immunodeficiencies, immunodiagnosis, immunogenetics, immunopathology, immunology of infectious disease and tumors, immunoprophylaxis including vaccine development and delivery, immunological aspects of pregnancy including passive immunity, autoimmuity, neuroimmunology, and transplanatation immunology. Manuscripts that describe new genes and development of tools such as monoclonal antibodies are also of interest when part of a larger biological study. Studies employing extracts or constituents (plant extracts, feed additives or microbiome) must be sufficiently defined to be reproduced in other laboratories and also provide evidence for possible mechanisms and not simply show an effect on the immune system.