Determination of trace fluoroquinolones in honey and milk based on cyclodextrin modified magnetic metal-organic frameworks solid phase extraction coupled with ultra-high performance liquid chromatography
Yangkun Sun , Jingjing Kuang , Yongzhe Cheng , Chuhui Lin , Hongyang Zhang , Min Zhang , Fanghong Ning , Ping Hu
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引用次数: 0
Abstract
Long-term intake of animal-derived foods with excessive fluoroquinolones (FQs) will cause damage to human health, so it is critical to establish a feasible approach for sensitive and rapid monitoring of FQs residues. In this study, a new cyclodextrin modified magnetic metal-organic frameworks (Fe3O4@UiO-66-CD) was successfully synthesized by amidation reaction and applied to magnetic solid phase extraction (MSPE) for FQs analysis. The adsorption behavior of Fe3O4@UiO-66-CD was consistent with the pseudo-second-order kinetics and Freundlich isothermal adsorption model, which indicated that the designed material had various interactions on FQs, such as host-guest interaction and π-π interaction. The parameters of MSPE were optimized and the determination method of norfloxacin, enrofloxacin, lomefloxacin and gatifloxacin was established by using MSPE combined with ultra-high performance liquid chromatography (UHPLC) and fluorescence detector (FLD). The method validation results displayed that the detection limits were 0.02–0.09 ng/mL, and the RSDs of intra-day and inter-day precision were less than 4.1 and 6.4 %, respectively. In the target FQs analysis of real honey and milk samples, the recoveries at different fortified concentrations were in the ranges of 88.4 % to 108.6 % with RSD ≤ 5.7 %. The results showed that the proposed method was sensitive, accurate and reliable for the determination of trace FQs in animal-derived foods.
期刊介绍:
The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.