Bioanalysis of six antibiotics from volumetric microsamples: a new tool for precision dosing in critically ill children.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-01-01 Epub Date: 2023-11-22 DOI:10.4155/bio-2023-0171

John Takyi-Williams, Abbie D Leino, Ruiting Li, Kevin J Downes, Athena F Zuppa, Amanda Bwint, Bo Wen, Duxin Sun, Marc H Scheetz, Manjunath P Pai

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引用次数: 0

Abstract

Background: Volumetric absorptive microsamples (VAMS) can support pharmacokinetic / pharmacodynamic studies. We present the bioanalytical method development for the simultaneous quantification of ampicillin, cefepime, ceftriaxone, meropenem, piperacillin, tazobactam, and vancomycin from VAMS. Methods & results: Optimal extraction, chromatographic, and mass spectrometry conditions were identified. Maximum extraction recoveries included 100 μl of water for rehydration and methanol for protein precipitation. Chromatographic separation used Phenomenex Kinetex^™ Polar C18 column with a mobile phase comprising water/acetonitrile with formic acid and was fully validated. Hematocrit effects were only observed for vancomycin. Samples were stable for 90 days at -80°C except for meropenem, which was stable for 60 days. Conclusion: Multiple antibiotics can be assayed from a single VAMS sample to facilitate pharmacokinetic/pharmacodynamic studies.

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体积微样品中六种抗生素的生物分析:一种用于危重儿童精确给药的新工具。

背景:体积吸收微样品(VAMS)可以支持药代动力学/药效学研究。我们介绍了同时定量氨苄西林、头孢吡肟、头孢曲松、美罗培南、哌拉西林、他唑巴坦和万古霉素的生物分析方法的发展。方法与结果:确定了最佳提取、色谱和质谱条件。最大提取回收率为复水合用水100 μl，蛋白质沉淀用甲醇100 μl。色谱分离使用Phenomenex Kinetex™Polar C18色谱柱，流动相为水/乙腈和甲酸，并经过充分验证。仅万古霉素对红细胞压积有影响。样品在-80℃下稳定90天，除美罗培南稳定60天外。结论:单一VAMS样品可检测多种抗生素，便于药代动力学/药效学研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.