Cellular polarity in cultured animal pole cells of Xenopus embryos

Journal of ultrastructure and molecular structure research Pub Date : 1989-12-01 DOI:10.1016/0889-1605(89)90021-9

Mari Asada-Kubota

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Abstract

The expression of intracellular and surface polarity in cultured animal pole cells of Xenopus embryos (stages 6, 8, and 10) was examined morphologically and immunocytochemically. When control embryos reached stage 23, daughter cells derived from a single or a few animal pole cells formed aggregates. Outer cells of the aggregates displayed intracellular and surface polarity and expressed an epidermis-specific antigen (XEPI-1) on the apical surface circumference, while these characteristics had not yet been established in the animal pole cells at the time of isolation. However, inner cells of the aggregates did not display the cellular polarity along an outer-inner axis of the aggregates and displayed the antigen randomly within the aggregates. These results indicate that the expression of cellular polarity in epidermal differentiation of Xenopus embryos in vitro depends on the position within the aggregates formed by daughter cells derived from isolated animal pole cells.

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爪蟾胚胎培养动物极细胞的细胞极性

用形态学和免疫细胞化学方法检测了培养的爪蟾胚胎(6、8和10期)动物极细胞的细胞内极性和表面极性的表达。当对照胚胎达到第23期时，由单个或几个动物极细胞衍生的子细胞形成聚集体。聚集体的外细胞表现出细胞内和表面极性，并在顶表面周长表达表皮特异性抗原(XEPI-1)，而这些特征在分离时尚未在动物极细胞中建立。然而，聚集体的内部细胞并没有沿着聚集体的内外轴显示细胞极性，而是在聚集体内随机显示抗原。这些结果表明，爪蟾胚胎表皮分化过程中细胞极性的表达取决于分离动物极细胞衍生的子细胞在聚集体中的位置。

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Journal of ultrastructure and molecular structure research

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