C Molinotti, P Puddu, S Perugini, P Barboni, R Biagi
{"title":"One-step purification by high-performance liquid chromatography of retinal S-antigen.","authors":"C Molinotti, P Puddu, S Perugini, P Barboni, R Biagi","doi":"10.1007/BF02871815","DOIUrl":null,"url":null,"abstract":"<p><p>A number of subsequent chromatographic techniques are commonly used, over a period of a few days, in order to obtain pure retinal S-antigen (S-Ag), a 50,000-dalton molecular weight protein with strong uveitopathogenic properties. A rapid high-performance liquid chromatographic technique employing a TSK-G3000 SWG gel-filtration column is described for the purification of bovine S-Ag. This single chromatographic step produced S-Ag preparations of high purity and recovery when compared to the conventionally isolated S-Ag.</p>","PeriodicalId":76092,"journal":{"name":"La Ricerca in clinica e in laboratorio","volume":"19 3","pages":"259-66"},"PeriodicalIF":0.0000,"publicationDate":"1989-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/BF02871815","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"La Ricerca in clinica e in laboratorio","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/BF02871815","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
A number of subsequent chromatographic techniques are commonly used, over a period of a few days, in order to obtain pure retinal S-antigen (S-Ag), a 50,000-dalton molecular weight protein with strong uveitopathogenic properties. A rapid high-performance liquid chromatographic technique employing a TSK-G3000 SWG gel-filtration column is described for the purification of bovine S-Ag. This single chromatographic step produced S-Ag preparations of high purity and recovery when compared to the conventionally isolated S-Ag.