Identification of immunoglobulin-containing cells in the central nervous system of the mouse following infection with the demyelinating strain of Semliki Forest virus.

L M Parsons, H E Webb
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Abstract

Cells within the central nervous system were identified as containing immunoglobulin G, A and M using immunocytochemistry in mice previously infected with Semliki Forest virus, a togavirus causing primary immune-mediated demyelination. Cells positive for these immunoglobulins were counted in cerebellar white matter, parenchyma, meninges and choroid plexus/ventricles. No positively staining cells were seen on day 6 after infection although other inflammatory cells were present at this time and virus-specific immunoglobulin was found in serum. Cells positive for IgG appeared in all areas by day 9 and remained dominant in numbers throughout. IgM-secreting cells appeared in small numbers in the parenchyma first on day 9 and subsequently in other areas, their numbers rising to a maximum on day 12 in all areas and falling thereafter. The number of IgA-secreting cells was small. They appeared by PID 12 and continued to rise on successive sampling days. Initially IgG-positive cells were seen in the perivascular cuffs but by day 12 a few had moved away from the cuffs into the adjacent parenchyma. IgG-positive cells were seen both in and away from cuffs within areas of demyelination. IgM and IgA-positive cells tended to follow the distribution of IgG-positive cells, but in fewer numbers.

塞姆利基森林病毒脱髓鞘株感染小鼠中枢神经系统含免疫球蛋白细胞的鉴定
在先前感染塞姆利基森林病毒(一种引起原发性免疫介导脱髓鞘的托加病毒)的小鼠中,使用免疫细胞化学方法鉴定出中枢神经系统内的细胞含有免疫球蛋白G、A和M。在小脑白质、实质、脑膜和脉络膜丛/脑室中检测到这些免疫球蛋白阳性的细胞。感染后第6天未见阳性染色细胞,但此时有其他炎症细胞存在,血清中发现病毒特异性免疫球蛋白。在第9天,IgG阳性细胞出现在所有区域,并在整个过程中保持优势。分泌igm的细胞在第9天开始少量出现在薄壁组织中,随后出现在其他区域,在第12天所有区域的分泌igm细胞数量达到最大值,随后下降。分泌iga的细胞数量较少。它们在PID 12出现,并在连续采样日持续上升。最初在血管周围袖口可见igg阳性细胞,但到第12天,一些细胞已从袖口转移到邻近的薄壁组织。在脱髓鞘区域的袖口内外均可见igg阳性细胞。IgM和iga阳性细胞倾向于跟随igg阳性细胞的分布,但数量较少。
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