Murine mercury-induced immune-complex disease: effect of cyclophosphamide treatment and importance of T-cells.

P Hultman, S Eneström
{"title":"Murine mercury-induced immune-complex disease: effect of cyclophosphamide treatment and importance of T-cells.","authors":"P Hultman,&nbsp;S Eneström","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The renal immune-complex (IC) disease induced in BALB/c mice by subcutaneous injection of mercuric chloride (1.6 mg/kg b.w.) every third day for 8 weeks was prevented by the intraperitoneal injection of cyclophosphamide (20 mg/kg b.w.) 24 h prior to mercury injection. The importance of T-cells in the induction of immune-complex disease was studied. BALB/c mice given drinking water containing 20 mg/l of HgCl2 for 10 weeks showed an increased titre of granular, mesangial IgG deposits and vessel wall IgG deposits. Identically treated, congenic nude BALB/c mice with a similar body burden of mercury developed no IC-disease. Cytophotometric analysis of the T-cell subsets in subcutaneously mercury-treated mice revealed a decrease in the fraction of T-helper (L3T4+) splenic cells in the SJL and C57BL/6J strains; no significant change in the T-cell subsets was found in BALB/c mice. C57BL/6J mice, resistant to induction of IC-disease by mercury, showed no increase in the fraction of T-suppressor/cytotoxic (Lyt-2+) cells and no change in the T-helper/T-suppressor cell ratio. C57BL/6J mice could not be rendered susceptible to mercury-induced IC-disease by treatment with different doses of cyclophosphamide.</p>","PeriodicalId":9248,"journal":{"name":"British journal of experimental pathology","volume":"70 3","pages":"227-36"},"PeriodicalIF":0.0000,"publicationDate":"1989-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2040569/pdf/brjexppathol00147-0005.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"British journal of experimental pathology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The renal immune-complex (IC) disease induced in BALB/c mice by subcutaneous injection of mercuric chloride (1.6 mg/kg b.w.) every third day for 8 weeks was prevented by the intraperitoneal injection of cyclophosphamide (20 mg/kg b.w.) 24 h prior to mercury injection. The importance of T-cells in the induction of immune-complex disease was studied. BALB/c mice given drinking water containing 20 mg/l of HgCl2 for 10 weeks showed an increased titre of granular, mesangial IgG deposits and vessel wall IgG deposits. Identically treated, congenic nude BALB/c mice with a similar body burden of mercury developed no IC-disease. Cytophotometric analysis of the T-cell subsets in subcutaneously mercury-treated mice revealed a decrease in the fraction of T-helper (L3T4+) splenic cells in the SJL and C57BL/6J strains; no significant change in the T-cell subsets was found in BALB/c mice. C57BL/6J mice, resistant to induction of IC-disease by mercury, showed no increase in the fraction of T-suppressor/cytotoxic (Lyt-2+) cells and no change in the T-helper/T-suppressor cell ratio. C57BL/6J mice could not be rendered susceptible to mercury-induced IC-disease by treatment with different doses of cyclophosphamide.

小鼠汞诱导的免疫复合物疾病:环磷酰胺治疗的效果和t细胞的重要性。
每隔3天皮下注射氯化汞(1.6 mg/kg b.w)诱发BALB/c小鼠肾免疫复合物(IC)疾病,连续8周,在注射汞前24小时腹腔注射环磷酰胺(20 mg/kg b.w)可预防这种疾病。研究了t细胞在免疫复合物疾病诱导中的重要性。饮用含20 mg/l HgCl2的水10周后,BALB/c小鼠颗粒状、系膜和血管壁IgG沉积滴度增加。同样处理,具有相似体汞负荷的基因裸BALB/c小鼠未发生ic疾病。皮下汞处理小鼠的t细胞亚群的细胞光度分析显示,SJL和C57BL/6J株的t辅助(L3T4+)脾脏细胞的比例降低;BALB/c小鼠的t细胞亚群未见明显变化。C57BL/6J小鼠抗汞诱导ic病,t抑制细胞/细胞毒性细胞(Lyt-2+)的比例没有增加,t辅助细胞/ t抑制细胞的比例没有变化。不同剂量的环磷酰胺均不能使C57BL/6J小鼠对汞诱导的ic病产生易感。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信