An electron microscope and immunohistochemical study of the intracellular location of Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis.

T A Sims, J Hay, I C Talbot
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引用次数: 0

Abstract

The wall of intact Toxoplasma tissue cysts within the brains of mice with congenital toxoplasmosis was investigated. Smaller cysts were identified within the soma of neurones. With larger cysts, the contained cystozoites were shown by ultrastructural examination to be surrounded by a layer of microtubules; immunohistochemical staining revealed that this layer contained neurofilament protein. Interior to this layer was a much convoluted parasitophorus vacuole membrane; exterior was the host cell membrane. In most cases, synaptic plates were noted on the outer plasma membrane. In no instance were tissue cysts observed either within neuroglial cells or in the absence of host cells. These observations are discussed in relation to the pathogenesis of congenital toxoplasmosis in the brain.

先天性弓形虫病小鼠脑内弓形虫组织囊肿细胞内定位的电镜和免疫组化研究。
研究了先天性弓形虫病小鼠脑内完整的弓形虫组织囊肿壁。在神经元胞体内发现较小的囊肿。在较大的囊肿中,超微结构检查显示囊子被一层微管包围;免疫组化染色显示该层含有神经丝蛋白。在这一层的内部是一个非常卷曲的寄虫液泡膜;外为宿主细胞膜。多数病例外质膜上可见突触板。在神经胶质细胞内或没有宿主细胞的情况下均未观察到组织囊肿。这些观察结果讨论了先天性脑弓形虫病的发病机制。
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