The IAA17.1/HSFA5a module enhances salt tolerance in Populus tomentosa by regulating flavonol biosynthesis and ROS levels in lateral roots

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2023-11-17 DOI:10.1111/nph.19382

Qin Song, Fu He, Lingfei Kong, Jiarui Yang, Xiaojing Wang, Zhengjie Zhao, Yuqian Zhang, Changzheng Xu, Chunfen Fan, Keming Luo

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引用次数: 0

Abstract

Auxin signaling provides a promising approach to controlling root system architecture and improving stress tolerance in plants. However, how the auxin signaling is transducted in this process remains unclear.
The Aux indole-3-acetic acid (IAA) repressor IAA17.1 is stabilized by salinity, and primarily expressed in the lateral root (LR) primordia and tips in poplar. Overexpression of the auxin-resistant form of IAA17.1 (IAA17.1m) led to growth inhibition of LRs, markedly reduced salt tolerance, increased reactive oxygen species (ROS) levels, and decreased flavonol content. We further identified that IAA17.1 can interact with the heat shock protein HSFA5a, which was highly expressed in roots and induced by salt stress. Overexpression of HSFA5a significantly increased flavonol content, reduced ROS accumulation, enhanced LR growth and salt tolerance in transgenic poplar. Moreover, HSFA5a could rescue the defective phenotypes caused by IAA17.1m.
Expression analysis showed that genes associated with flavonol biosynthesis were altered in IAA17.1m- and HAFA5a-overexpressing plants. Furthermore, we identified that HSFA5a directly activated the expression of key enzyme genes in the flavonol biosynthesis pathway, while IAA17.1 suppressed HSFA5a-mediated activation of these genes.
Collectively, the IAA17.1/HSFA5a module regulates flavonol biosynthesis, controls ROS accumulation, thereby modulating the root system of poplar to adapt to salt stress.

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IAA17.1/HSFA5a模块通过调节毛白杨侧根黄酮醇生物合成和ROS水平，增强毛白杨耐盐性。

生长素信号是调控植物根系结构和提高植物抗逆性的重要途径。然而，生长素信号在这一过程中是如何转导的尚不清楚。对吲哚-3-乙酸(IAA)抑制因子IAA17.1受盐度稳定，主要表达于侧根(LR)原基和叶尖。过表达抗生长素型IAA17.1 (IAA17.1m)导致LRs生长受到抑制，耐盐性显著降低，活性氧(ROS)水平升高，黄酮醇含量降低。我们进一步发现IAA17.1可以与盐胁迫诱导的根内高表达热休克蛋白HSFA5a相互作用。过表达HSFA5a显著提高转基因杨树黄酮醇含量，减少ROS积累，增强LR生长和耐盐性。此外，HSFA5a可以挽救iaa17.1引起的缺陷表型。表达分析表明，过表达IAA17.1m-和hafa5a的植物中与黄酮醇生物合成相关的基因发生了改变。此外，我们发现HSFA5a直接激活黄酮醇生物合成途径中关键酶基因的表达，而IAA17.1抑制HSFA5a介导的这些基因的激活。综上所述，IAA17.1/HSFA5a模块调节黄酮醇生物合成，控制ROS积累，从而调节杨树根系适应盐胁迫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.