Regulation of T16H subcellular localization for promoting its catalytic efficiency in yeast cells.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2024-02-01 Epub Date: 2023-11-16 DOI:10.1007/s10529-023-03442-3
Jiancong Cao, Bingrun Yang, Mengxia Zhang, Fang Yu
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引用次数: 0

Abstract

To investigate the effect of subcellular localization on the transformation efficiency of heterologous expressed functional P450s in yeast. Microbial biotransformation offers a promising substitute for the direct extraction of natural products, but its viability in industrial applications depends on achieving high transformation efficiencies. To investigate the influence of subcellular microenvironments on the activity of heterologously expressed P450s, Catharanthus roseus tabersonine 16-hydroxylase (T16H) was chosen, and its subcellular localization was regulated by fusing organelle-localization signals. Interestingly, this manipulation had no effect on the gene expression levels of T16H, but resulted in varying conversion rates from tabersonine to 16-hydroxy tabersonine. Notably, the highest transformation efficiency was observed in yeast cells expressing peroxisome-localized T16H. Given the alkaline pH optimum for P450s, the alkaline peroxisomal lumen could be a suitable compartment for P450s reactions to achieve high transformation efficiency using yeast cells. Different organelle-localization of T16H in yeast cells resulted in varying conversion rates, suggesting that compartmentalizing the expression of target enzymes could be a viable approach to increase transformation efficiency in yeast.

Abstract Image

调控T16H亚细胞定位促进其在酵母细胞中的催化效率。
目的研究亚细胞定位对外源表达功能p450在酵母中转化效率的影响。微生物转化为直接提取天然产物提供了一个有前途的替代品,但其在工业应用中的可行性取决于实现高转化效率。为了研究亚细胞微环境对P450s异源表达活性的影响,我们选择了花蔷薇tabersonine 16-羟化酶(T16H),并通过融合细胞器定位信号调控其亚细胞定位。有趣的是,这种操作对T16H的基因表达水平没有影响,但导致从tabersonine到16-羟基tabersonine的转化率不同。值得注意的是,在表达过氧化物酶体定位的T16H的酵母细胞中观察到最高的转化效率。考虑到p450的最佳碱性pH,碱性过氧化物酶体管腔可以作为p450反应的合适腔室,以实现酵母细胞的高转化效率。酵母细胞中不同的T16H细胞器定位导致了不同的转化率,表明区隔化靶酶的表达可能是一种提高酵母转化效率的可行方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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