Electron Paramagnetic Resonance Applied to Free Radicals and Reactive Oxygen Species Detection in Plant Systems

IF 1.1 4区物理与天体物理 Q4 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL

Applied Magnetic Resonance Pub Date : 2023-10-26 DOI:10.1007/s00723-023-01625-9

Francine A. F. Menezes, Jurandi G. Oliveira, André O. Guimarães

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引用次数: 0

Abstract

Electron paramagnetic resonance (EPR) is a non-invasive and highly sensitive technique that has been extensively used for detecting free radicals and reactive oxygen species (ROS) in biological systems. In plant biology, ROS are highly reactive and play a critical role in regulating various cellular processes. However, detecting ROS in plant systems is challenging due to their short half-life and low concentrations. This review presents a comprehensive overview of the application of EPR for the detection of free radicals and ROS in plant systems, including a comparison with other methods. Furthermore, it explores the utilization of the spin trapping method in combination with EPR for ROS detection, providing spectroscopic parameters for numerous spin trap compounds. We highlight some of the relevant studies that used EPR spectroscopy to detect ROS, as well as stable radicals, in plant systems. This review serves as a valuable resource for researchers interested in using EPR spectroscopy for free radicals and ROS detection in plant systems and provides insights into the current state of the field and future research trends.

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电子顺磁共振应用于植物系统中的自由基和活性氧检测

电子顺磁共振（EPR）是一种非侵入性的高灵敏度技术，已被广泛用于检测生物系统中的自由基和活性氧（ROS）。在植物生物学中，ROS 具有高活性，在调节各种细胞过程中发挥着关键作用。然而，由于 ROS 的半衰期短、浓度低，在植物系统中检测 ROS 具有挑战性。本综述全面概述了 EPR 在植物系统中用于检测自由基和 ROS 的应用，包括与其他方法的比较。此外，它还探讨了如何将自旋捕获法与 EPR 结合用于 ROS 检测，并提供了许多自旋捕获化合物的光谱参数。我们重点介绍了一些利用 EPR 光谱检测植物系统中 ROS 以及稳定自由基的相关研究。本综述为有兴趣在植物系统中使用 EPR 光谱检测自由基和 ROS 的研究人员提供了宝贵的资源，并为该领域的现状和未来研究趋势提供了见解。

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来源期刊

Applied Magnetic Resonance 物理-光谱学

CiteScore

1.90

自引率

10.00%

发文量

审稿时长

2.3 months

期刊介绍： Applied Magnetic Resonance provides an international forum for the application of magnetic resonance in physics, chemistry, biology, medicine, geochemistry, ecology, engineering, and related fields. The contents include articles with a strong emphasis on new applications, and on new experimental methods. Additional features include book reviews and Letters to the Editor.