Ameliorative Effects of Vitamins C and E on Haematotoxicity and Spleen Histopathology Induced by Dichlorvos Insecticide in Female Wistar Rats

Halima Mfaume, Harishchandra Pratap, Claudius Luziga, Felister Urasa
{"title":"Ameliorative Effects of Vitamins C and E on Haematotoxicity and Spleen Histopathology Induced by Dichlorvos Insecticide in Female Wistar Rats","authors":"Halima Mfaume, Harishchandra Pratap, Claudius Luziga, Felister Urasa","doi":"10.4314/tjs.v49i2.21","DOIUrl":null,"url":null,"abstract":"This research aimed to assess the toxicological effects of dichlorvos insecticide (2,2-dichlorovinyl dimethyl phosphate or DDVP) on haematological indices of female Wistar rats and to investigate the antioxidant properties of vitamins C and E in ameliorating haematotoxicity. The rats were divided into 6 groups; control-water, control-oil, dichlorvos group, dichlorvos + vitamin C group, dichlorvos + vitamin E group and dichlorvos + vitamin C + E group. Dichlorvos and vitamins were orally administered to rats with an interval of half an hour between treatments. The control-oil group was given 2 ml corn oil and the control-water group was given water adlib. The treatments were done for 28 consecutive days and blood samples were taken by cardiac puncture. The following haematological parameters were analysed: RBC, WBC, Hb, PCV, MCV, MCH, MCHC and THR. Bone marrow smears were prepared on 14th and 28th days for various blood stem cells evaluation and spleen tissues for histopathology. There was a significant decrease (p ˂ 0.01) in RBC counts, PCV, MCV and MCH values, whereas the WBC, THR counts and MCHC values increased significantly (p ˂ 0.01) in dichlorvos treated rats as compared to the controls and dichlorvos + vitamins co-treated groups. There were no significant differences in blood parameter counts between controls and dichlorvos + vitamins co-treated groups. In the bone marrow smear of the dichlorvos treated group, there was increased number of megakaryocytes and mature neutrophils. In conclusion, findings from the current study revealed that, vitamins C and E supplements were capable of mitigating the haematotoxic effects induced by dichlorvos insecticide in Wistar rats.","PeriodicalId":22207,"journal":{"name":"Tanzania Journal of Science","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tanzania Journal of Science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/tjs.v49i2.21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

This research aimed to assess the toxicological effects of dichlorvos insecticide (2,2-dichlorovinyl dimethyl phosphate or DDVP) on haematological indices of female Wistar rats and to investigate the antioxidant properties of vitamins C and E in ameliorating haematotoxicity. The rats were divided into 6 groups; control-water, control-oil, dichlorvos group, dichlorvos + vitamin C group, dichlorvos + vitamin E group and dichlorvos + vitamin C + E group. Dichlorvos and vitamins were orally administered to rats with an interval of half an hour between treatments. The control-oil group was given 2 ml corn oil and the control-water group was given water adlib. The treatments were done for 28 consecutive days and blood samples were taken by cardiac puncture. The following haematological parameters were analysed: RBC, WBC, Hb, PCV, MCV, MCH, MCHC and THR. Bone marrow smears were prepared on 14th and 28th days for various blood stem cells evaluation and spleen tissues for histopathology. There was a significant decrease (p ˂ 0.01) in RBC counts, PCV, MCV and MCH values, whereas the WBC, THR counts and MCHC values increased significantly (p ˂ 0.01) in dichlorvos treated rats as compared to the controls and dichlorvos + vitamins co-treated groups. There were no significant differences in blood parameter counts between controls and dichlorvos + vitamins co-treated groups. In the bone marrow smear of the dichlorvos treated group, there was increased number of megakaryocytes and mature neutrophils. In conclusion, findings from the current study revealed that, vitamins C and E supplements were capable of mitigating the haematotoxic effects induced by dichlorvos insecticide in Wistar rats.
维生素C和E对敌敌畏杀虫剂致雌性Wistar大鼠血液毒性和脾脏组织病理学的改善作用
本研究旨在评价敌敌畏杀虫剂(2,2-二氯酰磷酸二甲酯或DDVP)对雌性Wistar大鼠血液学指标的毒理学影响,并探讨维生素C和E对血液毒性的改善作用。将大鼠分为6组;对照水、对照油、敌敌畏组、敌敌畏+维生素C组、敌敌畏+维生素E组、敌敌畏+维生素C + E组。对大鼠口服敌敌畏和维生素,每隔半小时给药。对照组给予玉米油2 ml,对照组给予水adlib。连续治疗28天,穿刺取血。分析以下血液学参数:RBC、WBC、Hb、PCV、MCV、MCH、MCHC和THR。第14天和第28天分别进行骨髓涂片,用于各种造血干细胞评估和脾脏组织病理学检查。与对照组和敌敌畏+维生素共处理组相比,敌敌畏处理大鼠红细胞计数、PCV、MCV和MCH值显著降低(p小于0.01),而WBC、THR计数和MCHC值显著升高(p小于0.01)。对照组与敌敌畏+维生素联合处理组的血液参数计数无显著差异。敌敌畏处理组骨髓涂片中巨核细胞和成熟中性粒细胞增多。综上所述,本研究结果表明,维生素C和维生素E补充剂能够减轻敌敌畏杀虫剂对Wistar大鼠的血液毒性作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信