Testicular function of sexually immature rats chronically treated with melatonin.

A N Olivares, L E Valladares, E Bustos-Obregón, S M Núñez
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Abstract

Melatonin disturbs the reproductive process in seasonal but not in continuous breeders: however, it delays sexual development in the rat. The testicular function of rats injected daily with melatonin from 20 up to 25, 30, 35 or 40 days of age was analyzed. The spermatogenic and androgenic activity of testes was assessed by light microscopy and by the capacity for binding hCG and producing testosterone in vitro, respectively. In addition, LH, FSH and testosterone plasma levels were measured and 3B-hydroxysteroid dehydrogenase activity of Leydig cells was assessed histochemically to aid in the interpretation of results. Rats treated with melatonin for 15 or 20 days presented at the end of the juvenile period, abnormal progression of spermatogenesis and decreased ability of their Leydig cells to produce testosterone both in vivo and in vitro. This was associated with a lower number of binding sites for hCG and diminished production of testosterone in response to receptor and post-receptor mediated stimulation of steroidogenesis. Melatonin caused a marked decrease in LH serum levels. The diminished LH supply to the testis probably interfered with differentiation or impaired the functional ability of Leydig cells. As a consequence, testicular testosterone production was insufficient to support normal spermatogenic progression and growth and development of the sexual accessory organs.

长期服用褪黑素对性成熟大鼠睾丸功能的影响。
褪黑素会干扰季节性繁殖鼠的生殖过程,但不会干扰连续繁殖鼠的生殖过程;然而,褪黑素会延迟大鼠的性发育。对20 ~ 25、30、35、40日龄每日注射褪黑素的大鼠睾丸功能进行了分析。在体外分别通过光镜和结合hCG和产生睾酮的能力来评估睾丸的生精活性和雄激素活性。此外,测定血浆LH、FSH和睾酮水平,组织化学评估间质细胞3b -羟基类固醇脱氢酶活性,以帮助解释结果。用褪黑激素治疗15或20天的大鼠,在幼年期结束时,精子发生异常进展,体内和体外的间质细胞产生睾酮的能力下降。这与hCG结合位点数量减少以及响应受体和受体后介导的类固醇生成刺激而减少睾酮的产生有关。褪黑素导致黄体生成素血清水平显著下降。睾丸LH供应的减少可能干扰了睾丸间质细胞的分化或损害了其功能能力。结果,睾丸睾酮的产生不足以支持正常的生精进程和性附属器官的生长发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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