Pulmonary response to free fatty acid intravenous infusion in the rabbit: role of leukotrienes and the effect of prostacyclin.

G Arenas, R Del Buono, M J Oyarzún, P Donoso, D Quijada
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Abstract

Intravenous infusion of free fatty acid (FFA) 20 mg.kg-1.min-1 produces pulmonary edema, hypoxemia, hyperventilation and increase in the alveolar surfactant content in rabbits in less than 15 min. We tried to study the role of leukotrienes (LT) and the effects of PGI2 in pulmonary response to FFA. We used Piriprost an inhibitor of LT synthesis or Epoprostenol (Prostacyclin: PGI2) in 4 series of rabbits treated with FFA or its vehicle. Piriprost given as an aerosol (0.1% W/W in THAM) scarcely modified the morphofunctional changes induced by FFA. The only pulmonary effect prevented by Piriprost was the increase in surfactant content (disaturated phosphatidylcholine: DSPC) in broncho-alveolar lavage gluid (BAL). PGI2 administered in a dose of 0.1 micrograms.kg-1.min-1 5 minutes prior to a 15 min infusion of FFA was also unable to prevent most of the effects of FFA on the lung. Only the increase in DSPC in BAL was prevented by PGI2. Some animals received a smaller dose of FFA, because they died earlier. Piriprost, as well as PGI2, shortened the survival time of rabbits treated with FFA. This decrease in the survival rate of animals treated with FFA could account for the lack of increase in DSPC post-FFA. Since other morphofunctional changes induced by FFA were scarcely modified by both Piriprost or PGI2, our results suggest that it is unlikely that either leukotrienes on PGI2 may have a significant effect on pulmonary disturbances induced by FFA.

兔肺对游离脂肪酸静脉输注的反应:白三烯的作用和前列环素的作用。
静脉滴注游离脂肪酸(FFA) 20 mg.kg-1。min-1可在不到15分钟的时间内引起家兔肺水肿、低氧血症、过度通气和肺泡表面活性剂含量升高。我们试图研究白三烯(LT)和PGI2在肺对FFA反应中的作用。我们用吡前列素(LT合成抑制剂)或环丙烯醇(环丙素:PGI2)治疗4组经FFA或其载体处理的家兔。吡前列素作为气溶胶给药(THAM中0.1% W/W)几乎没有改变FFA诱导的形态功能变化。匹利前列素唯一阻止肺功能的是支气管肺泡灌洗液(BAL)中表面活性剂含量(不饱和磷脂酰胆碱:DSPC)的增加。PGI2给药剂量为0.1微克。kg-1。在15分钟输注FFA前的1 - 5分钟也无法阻止FFA对肺的大部分影响。PGI2仅能阻止BAL中DSPC的增加。一些动物接受的FFA剂量较小,因为它们死得早。吡前列素和PGI2均可缩短FFA处理家兔的存活时间。用FFA治疗的动物存活率的下降可以解释FFA后dsc缺乏增加的原因。由于由FFA引起的其他形态功能变化几乎没有被匹利前列素或PGI2改变,我们的研究结果表明,PGI2上的白三烯不太可能对由FFA引起的肺紊乱有显著影响。
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