{"title":"EXCEPTIONAL ANTIOXIDANT EFFECT OF URTICA DIOICA EXTRACT ON MOTILITY AND SOME OTHER POST THAWED GOAT SEMEN CHARACTERISTICS","authors":"K. AL-JAF, C. OMAR, A. VAZIRY","doi":"10.15666/aeer/2105_38653885","DOIUrl":null,"url":null,"abstract":". This study was carried out to examine the effects of Urtica dioica extract on goat cryopreserved semen quality. The ejaculates of Markhoz goats (n = 12) were collected by an electro-ejaculator. The semen was diluted and supplemented with different concentration of aqueous or hydroalcoholic Urtica dioica extract of aerial or root parts as follows: aerial-aqueous (AA1 5μg/ml , AA2 15μg/ml and AA3 45μg/ml ); aerial-ethanolic (AE1 5μg/ml , AE2 15μg/ml and AE3 45μg/ml ); root aqueous (RA1 5μg/ml , RA2 15μg/ml and RA3 45μg/ml ); root-ethanolic (RE1 5μg/ml , RE 215μg/ml and RE3 45μg/ml ) and control (CCC 0mg/ml ) without additives. After thawing, sperm samples were subjected to viability, acrosome integrity, plasma membrane integrity, motility, sperm chromatin dispersion (SCD) and concentration of malondialdehyde (MDA) assays. Addition of AA1 5μg/ml to the extender significantly (P < 0.05) improved progressive motility, viability, plasma membrane integrity, acrosome integrity, and DNA integrity of the semen samples. Sperm viability and plasma membrane integrity in aqueous extract (both aerial & root parts)-supplemented extenders were relatively higher, as compared to the control. Moreover, a significant decrease in MDA concentration was detected in semen supplemented with Urtica dioica extract treatments. In conclusion, the results indicate that 5μg/ml of aqueous extract of Urtica dioica aerial part improved sperm quality and could be recommended as an additive to the extender for cryopreservation. This study shows a novel approach in goat semen cryopreservation due to the remarkable influence of Urtica dioica on post-thawed sperm parameters.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"97 1","pages":"0"},"PeriodicalIF":0.6000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Ecology and Environmental Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15666/aeer/2105_38653885","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
. This study was carried out to examine the effects of Urtica dioica extract on goat cryopreserved semen quality. The ejaculates of Markhoz goats (n = 12) were collected by an electro-ejaculator. The semen was diluted and supplemented with different concentration of aqueous or hydroalcoholic Urtica dioica extract of aerial or root parts as follows: aerial-aqueous (AA1 5μg/ml , AA2 15μg/ml and AA3 45μg/ml ); aerial-ethanolic (AE1 5μg/ml , AE2 15μg/ml and AE3 45μg/ml ); root aqueous (RA1 5μg/ml , RA2 15μg/ml and RA3 45μg/ml ); root-ethanolic (RE1 5μg/ml , RE 215μg/ml and RE3 45μg/ml ) and control (CCC 0mg/ml ) without additives. After thawing, sperm samples were subjected to viability, acrosome integrity, plasma membrane integrity, motility, sperm chromatin dispersion (SCD) and concentration of malondialdehyde (MDA) assays. Addition of AA1 5μg/ml to the extender significantly (P < 0.05) improved progressive motility, viability, plasma membrane integrity, acrosome integrity, and DNA integrity of the semen samples. Sperm viability and plasma membrane integrity in aqueous extract (both aerial & root parts)-supplemented extenders were relatively higher, as compared to the control. Moreover, a significant decrease in MDA concentration was detected in semen supplemented with Urtica dioica extract treatments. In conclusion, the results indicate that 5μg/ml of aqueous extract of Urtica dioica aerial part improved sperm quality and could be recommended as an additive to the extender for cryopreservation. This study shows a novel approach in goat semen cryopreservation due to the remarkable influence of Urtica dioica on post-thawed sperm parameters.
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