Xpert MTB/RIF assay among the extra-pulmonary and non-sputum respiratory samples: One year experience at National Tuberculosis Reference Laboratory, Bangladesh

Bangladesh Journal of Medical Microbiology Pub Date : 2023-10-10 DOI:10.3329/bjmm.v17i1.68139

Adeeba Khanduker, Sadia Sharmin, Shamim Hossain, Tanvir Huda, Farzana Mahzabin Monika, Elma Mostofa, S M Mostofa Kamal, Afroza Begum, Md Roknuzzaman Khan, S M Abdur Razzaque, Pronab Kumar Modak, Md Shirajul Islam

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Abstract

Background: Extra-pulmonary tuberculosis (EPTB) is a diagnostic and therapeutic challenge due to the paucity of suitable rapid diagnostic tools. Objectives: The aim was to assess the frequency of detection of Mycobacterium tuberculosis using Xpert MTB /RIF assay among the extra-pulmonary and non-sputum respiratory samples (NSRS) along with demographic profile, life style variables and proportion of Rifampicin resistant TB among those specimens. Methodology: This cross-sectional retrospective study was conducted at National Tuberculosis Reference Laboratory (NTRL) from January 2018 to December 2018 for period of 1 year. Total 3918 extra-pulmonary and non-sputum respiratory samples from suspected TB patients were enrolled. Detection of Mycobacterium tuberculosis and Rifampicin resistance was performed by Xpert MTB /RIF assay and culture in both liquid and solid media. Results: Among 3918 samples, 3152 were new cases and 766 were previously treated TB cases. A total of 853 (21.77%) MTB were detected by Xpert MTB /RIF assay. Among them, 713 (83.59%) were extra-pulmonary and 140 (16.41%) were non-sputum respiratory samples. Lymph node (LN) aspirates and tissues were the most frequent (37.9 and 9.9%) samples of EPTB. The proportion of RR cases among EPTB was 2.8% and 6.3% among new and previously treated cases. And 2.6% and 11.1% among NSRS new and previously treated cases respectively. Conclusion: This study will give an insight of EPTB and NSRS profile with a view to diagnostic and therapeutic approach in Bangladesh. Bangladesh Journal of Medical Microbiology, January 2023;17(1):21-28

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肺外和非痰呼吸道样本中的MTB/RIF检测专家:孟加拉国国家结核病参考实验室一年经验

背景:由于缺乏合适的快速诊断工具，肺外结核(EPTB)是一个诊断和治疗的挑战。目的:评估Xpert MTB /RIF法在肺外和非痰呼吸样本(NSRS)中检测结核分枝杆菌的频率，以及这些样本中人口统计资料、生活方式变量和利福平耐药结核病的比例。方法:本横断面回顾性研究于2018年1月至2018年12月在国家结核病参考实验室(NTRL)进行，为期1年。共纳入疑似结核病患者的3918份肺外和非痰呼吸道样本。采用Xpert MTB /RIF法检测结核分枝杆菌和利福平耐药性，并进行液体和固体培养基培养。结果:3918例病例中，新发病例3152例，既往结核病例766例。Xpert MTB /RIF法共检出MTB 853株(21.77%)。其中肺外标本713例(83.59%)，非痰呼吸道标本140例(16.41%)。淋巴结(LN)吸出物和组织是EPTB最常见的样本(37.9%和9.9%)。在EPTB新发和既往治疗病例中，RR病例所占比例分别为2.8%和6.3%。在NSRS新发病例和既往治疗病例中分别占2.6%和11.1%。结论:本研究将为孟加拉国EPTB和NSRS的诊断和治疗方法提供见解。孟加拉医学微生物学杂志，2023年1月;17(1):21-28

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Bangladesh Journal of Medical Microbiology

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