Ali Hajibeglou, Mohamad Sudagar, Fatemeh Azizinezhad Jolandan, Kimia Alaei
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引用次数: 0
Abstract
The use of synthetic steroids for sex reversal in various fish is widespread, but due to the potential dangers of these types of compounds, the use of phytochemicals has been explored as a suitable natural alternative. In this experiment, the effects of extracts of Mucuna pruriens seeds and Basella alba leaves on sex reversal in Oncorhynchus mykiss were investigated. Fifteen-days-old rainbow trout larvae (mean weight: 0.123 ± 0.001 g) were fed experimental diets containing 17 α-methyltestosterone (MT) (60, 90 mg/kg diet), ethanol extract of B. alba leaves (1.0, 2.0 g/kg diet), and methanol extract of M. pruriens seeds (0.1, 0.2 g/kg diet) for 90 days. After 90 days, the experimental diets were replaced with the basal diet until 10 months of age. The control group was fed the basal diet. At the end of the experiment, growth factors, survival (%), intersex (%), and masculinization (%) were measured. The feed conversion ratio and specific growth rate in all experimental groups were not significantly different from those in the control group ( ). The highest significant percentage of intersex (6.03 ± 3.53%) and the lowest percentage of survival (72.33 ± 2.51%) were observed in the treatment with 90 mg MT/kg compared with the other treatments ( ). The percentage of intersexuality was significantly lower in the groups fed with plant extract than in the groups fed with MT ( ). The percentage of intersexuality was significantly lower in the groups fed with plant extract than in the groups fed with MT. The highest percentage of masculinization was observed in the groups fed with M. pruriens extract (0.2 g/kg) (61.75 ± 17.16%) and MT (90 mg/kg) (68.62 ± 1.82%). Moreover, masculinization was significantly higher in all experimental groups than in the control group ( ). There were significantly more males (%) in all experimental groups than in the control group. In conclusion, extracts of M. pruriens and B. alba can be used as an environmentally friendly and safe alternative to MT to induce masculinization of rainbow trout.
期刊介绍:
International in perspective, Aquaculture Research is published 12 times a year and specifically addresses research and reference needs of all working and studying within the many varied areas of aquaculture. The Journal regularly publishes papers on applied or scientific research relevant to freshwater, brackish, and marine aquaculture. It covers all aquatic organisms, floristic and faunistic, related directly or indirectly to human consumption. The journal also includes review articles, short communications and technical papers. Young scientists are particularly encouraged to submit short communications based on their own research.