Gas chromatography- mass spectrometry analysis of natural products in Pimpinella olivieri from North Iraq

Abstract

Wild plants possess many bioactive ingredients derived from by-metabolic process, which have a wide broad uses as medication and pharmaceutical importance. Therefore, the aim of the present study on a species of genus Pimpinella that belong to the family Apiaceae (Umbelliferae) developing northern Iraq Pimpinella olivieri , is the qualitative analysis of the methanolic extract of the plant to find out the active compounds in it by gas chromatographic technique equipped with mass spectroscopy. The results of the extract showed the presence of phenols, alkaloids, steroids, flavonoids, glycosides, fatty acids, terpenoids and carbohydrates. The results of GC-Mass analysis revealed presence of 20 biological compounds in the methanolic extract of the plant, such as sesquiterpenoid, lipophilic compounds. Bisbollene, Cis-13-octadecenoic acid, methyl ester (highest percentage of 72.05and 5.14) % at 15.1 and 24 minutes respectively of the detention time and the proportions of the rest of the terpenes in the plant extract were varied. The Benzene methanol was phenolic compound, 3,5-dimethyl, in ratio 12.96 at 13.3 minutes of detention time. While the two compounds (1,3,5,7-tetraethyl-1-methox cycl tetra siloxane and Cyclohexane, 1R-acetamido-2cis,4trans-bis(acetoxy)-3trans- azido) gave the lowest percentage of 0.13%.