Phosphocreatine and pH recovery without restoration of mechanical function during prolonged activity of rat gastrocnemius muscle: an in vivo 31P NMR study.

E Le Rumeur, L Le Moyec, F Chagneau, M Levasseur, P Toulouse, R Le Bars, J de Certaines
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引用次数: 5

Abstract

Metabolic impairment in skeletal muscle was suggested to be involved in the development of local mechanical fatigue but until now results have dealt with short activity periods whereas little data on exhaustive and prolonged exercises are available. Stimulations of rat leg muscle lasting 45 min were induced by tetanic trains delivered via sciatic nerve at five different rhythms. Energy metabolism of the stimulated gastrocnemius muscle was followed by 31P NMR spectroscopy using surface coil while mechanical function was recorded. Our data showed a decrease in the force level to very low values a few minutes after exercise onset. This mechanical impairment only induced a transient metabolic failure followed by rapid restoration of high phosphocreatine (PCr) values and intracellular pH, without mechanical recovery. In addition, at the end of exercise, the PCr content was proportional to the fatigue level. As these experiments could not have impaired neuromuscular junction, the data would indicate that fatigue was maintained by a mechanism which does not appear to depend directly on muscle cell energy stores.

大鼠腓肠肌长时间活动过程中不恢复机械功能的磷酸肌酸和pH值恢复:一项体内31P核磁共振研究。
骨骼肌的代谢损伤被认为与局部机械疲劳的发展有关,但到目前为止,结果都是针对短时间的活动,而关于详尽和长时间运动的数据很少。通过坐骨神经以5种不同的节奏传递强直性电击,对大鼠腿部肌肉进行持续45分钟的刺激。采用表面线圈31P核磁共振波谱法观察受刺激腓肠肌的能量代谢,并记录其力学功能。我们的数据显示,在运动开始几分钟后,力水平下降到非常低的值。这种机械损伤只引起短暂的代谢衰竭,随后迅速恢复高磷酸肌酸(PCr)值和细胞内pH值,没有机械恢复。此外,运动结束时,PCr含量与疲劳程度成正比。由于这些实验不可能损害神经肌肉连接,数据将表明疲劳是由一种机制维持的,这种机制似乎并不直接依赖于肌肉细胞的能量储存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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