Comparison of malondialdehyde, hydroxynonenal, isoprostane and 8-hydroxydeoxyguanosine in osteoarthritis patients and healthy controls

JAIMC Journal of Allama Iqbal Medical College Pub Date : 2023-10-28 DOI:10.59058/jaimc.v21i3.113

Zoya Muzzafar, Mehreen Zaidi, None Shazia Ramzan, Neelum Bilal, Tania Ahmad Shakori, Fahim Hadi, Tahir Maqbool, Muhammad Tahir, Shabana Akhtar

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Abstract

Abstract Objective: To study the level of oxidative Stress markers like malondialdehyde, 4 Hydroxynonenal (HNE), Isoprostane and 8 hydroxyguanosine was measured in the serum, saliva and urine of the patients suffering from osteoarthritis. Methods: In this study, oxidative stress markers in the body fluids of Osteoarthritic( OA) patients were compared with the control groups. The level of stress markers were determined by commercially available quantitative ELISA kits. Result: Our findings indicate that the highest levels of these oxidative stress markers were detected in serum followed by urine. The saliva overall presented less levels of oxidative stress markers in individuals with and without OA. In addition to this detection of MDA in serum sample was found to be the most important tool in the detection of OA. Conclusion: the clinical significance of biomarkers of oxidative stress in humans must come from a critical analysis of the markers that should give an overall index of redox status in particular conditions. Keywords: Osteoarthritis, MDA, HNE, 8-Hydroxyguanosine, Isoprostane, lipidperoxidation

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骨关节炎患者与健康对照丙二醛、羟基壬烯醛、异前列腺素和8-羟基脱氧鸟苷的比较

文摘& # x0D;目的:研究骨关节炎患者血清、唾液和尿液中丙二醛、4羟基壬烯醛(HNE)、异前列腺素和8羟基鸟苷等氧化应激标志物的水平。方法:将骨关节炎(OA)患者体液中的氧化应激标志物与对照组进行比较。采用市售定量ELISA试剂盒检测应激标志物水平。结果:我们的研究结果表明，这些氧化应激标志物在血清中检测到最高水平，其次是尿液。在有OA和没有OA的个体中，唾液总体上呈现出较低水平的氧化应激标志物。此外，血清样品中MDA的检测被认为是检测OA最重要的工具。结论:人类氧化应激生物标志物的临床意义必须来自对这些标志物的批判性分析，这些标志物应该在特定条件下给出氧化还原状态的总体指标。关键词:骨关节炎，丙二醛，HNE, 8-羟基鸟苷，异前列腺素，脂质过氧化

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JAIMC Journal of Allama Iqbal Medical College

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