Assessment of the relationship between miR-499C/T (rs3746444) polymorphism and lung carcinoma in Iranian population; a case-control study

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL

African Health Sciences Pub Date : 2023-10-11 DOI:10.4314/ahs.v23i3.36

Mehdi Torabi, Mostafa Khafaei, Behnaz Jahanbin, Morteza Sadeghi

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引用次数: 0

Abstract

Introduction: Lung carcinoma is characterized by uncontrollable division of respiratory system cells with detrimental and lethalconsequences on human health. Critical roles of microRNAs (miR) are scientifically approved in biological and pathologicalpathways, such as the role of miR-499 (rs3746444) in lung carcinomas. Thus, in this case-control investigation, we aimed toassess the probable relationship between miR-499C/T variant and the occurrence of lung carcinoma in Iranian population forthe first time. Methods: Genotype of miR-499 polymorphism was described by the Polymerase Chain Reaction-Restriction Fragment LengthPolymorphism (PCR-RFLP) assay in patients and healthy individuals. Following definite diagnosis of lung carcinoma, the bloodsamples were collected, and the DNA extraction was performed by Salting-Out method. Finally, data were analysed by SPSS (v.20) and the significant level was considered p-value<0.05. Results: Statistically, the frequency of combined genotypes of CC+CT were 83.33% and 35% and TT+CT were 100% and 92%in case and control individuals, respectively. Also, individuals with genotypes of TC (OR: 3.08, CI95%: 3.03-3.17, p<0.0001),TC+CC (OR: 0.10, CI95%: 0.05-0.23, p<0.0001), CC (OR: 0, CI95%: 0.00-0.60, p=0.0214), and TC (OR: 0.07, CI95%: 0.03-0.15, p<0.0001) represented statistically significant (p<0.05) differences lung carcinoma than those with TT, TT, TT+TC, andTT+CC genotypes, respectively. The frequency of miR-499C (78.5%) and miR-499T (21.5%) alleles were also statistically significantly(p<0.05) difference associated with lung carcinoma in patients than controls. Conclusion: In this study, a possible relationship among miR-499C/T polymorphism and lung carcinoma was detected in Iranianpopulation. Since this study was conducted for the first time, thus other supplementary assessments are needed for definiteconclusion. Keywords: Lung, neoplasm; carcinoma; rs3746444; miR-499C/T; miR-499A/G; RFLP-PCR; Polymorphism; Iran.

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伊朗人群miR-499C/T (rs3746444)多态性与肺癌的关系病例对照研究

肺癌的特点是呼吸系统细胞分裂不可控，对人体健康具有有害和致命的后果。microRNAs (miR)在生物学和病理途径中的关键作用已被科学认可，例如miR-499 (rs3746444)在肺癌中的作用。因此，在这项病例对照研究中，我们旨在首次评估mir - 49c /T变异与伊朗人群肺癌发生之间的可能关系。方法:采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法对患者和健康人的miR-499多态性进行基因型分析。确诊肺癌后，采集血样，用盐析法提取DNA。最后，使用SPSS (v.20)对数据进行分析，认为p值为0.05。结果:病例组和对照组CC+CT合并基因型频率分别为83.33%和35%，TT+CT合并基因型频率分别为100%和92%。TC基因型(OR: 3.08, CI95%: 3.03-3.17, p<0.0001)、TC+CC基因型(OR: 0.10, CI95%: 0.05-0.23, p<0.0001)、CC基因型(OR: 0, CI95%: 0.00-0.60, p=0.0214)、TC基因型(OR: 0.07, CI95%: 0.03-0.15, p<0.0001)与TT、TT、TT+TC和TT+CC基因型相比，肺癌的发生差异具有统计学意义(p<0.05)。肺癌患者与对照组相比，miR-499C(78.5%)、miR-499T(21.5%)等位基因频率差异也有统计学意义(p < 0.05)。结论:本研究发现miR-499C/T多态性与伊朗人群肺癌之间可能存在关联。由于本研究为首次开展，因此需要其他补充评价才能明确结论。关键词:肺;肿瘤;癌;rs3746444;mir - 499 c / T;mir - 499 a / G;RFLP-PCR;多态性;伊朗。

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来源期刊

African Health Sciences MEDICINE, GENERAL & INTERNAL-

CiteScore

2.30

自引率

0.00%

发文量

179

审稿时长

>12 weeks

期刊介绍： The African Health Sciences is an internationally refereed journal publishing original articles on research, clinical practice, public health, policy, planning, implementation and evaluation, in the health and related sciences relevant to Africa and the tropics. Its objectives are to: Advocate for and promote the growth of reading culture in sub Saharan Africa; Provide a high quality journal in which health and policy and other researchers and practitioners in the region can and world wide, can publish their work; Promote relevant health system research and publication in the region including alternative means of health care financing, the burden of and solution of health problems in marginalized urban and rural communities amongst the displaced and others affected by conflict; Promote research and the systematic collection and collation and publication of data on diseases and conditions of equity and influence; Promote development of evidence-based policies and guidelines for clinical, public health and other practitioners. African Health Sciences acknowledges support provided by the African Health Journals Partnership Project that is funded by the US National Institutes of Health (through the National Library of Medicine and the Fogarty International Center) and facilitated by the Council of Science Editors.