EFFECTS OF FEEDING A1 AND A2 COW MILK-BASED DIET ON BIOCHEMICAL PARAMETERS IN DIABETIC MICE MODEL

Abstract

– Streptozotocin is naturally occurring target specific alkylating agent derived from gram-positive bacterium Streptomyces achromogenes species that triggers an inflammatory mechanism leading to preferential destruction of pancreatic beta cells which is responsible for hyperglycemic states in experimental C-57 BL/6 mice. Cytotoxic effect of Streptozotocin is well established to build type 1 diabetes mellitus (T1DM) study model. We induced diabetes in C57 bl/6 mice by injecting Streptozotocin (dissolved in 0.1 M sodium citrate buffer at pH of 4.4) intraperitoneally at dose of 45mg per kg of body weight for consecutive 5 days. The mice were fed with A1A1 and A2A2 milk and their response was seen at 3 months with respect to their controls. A2A2 diet was not related to the induction of biochemical abnormalities while A1A1 and A1A2 diet have some association to cause some inflammatory reactions.