ARTIFICIAL INSEMINATION IN DEEP INTRACORNUAL FIXED TIME IN BOVINE

Abstract

– The search for improvements in the efficiency of the artificial insemination (AI) technique is still required. The objective of this study was to verify if intracornual fixed-time artificial insemination can improve conception rates concerning transcervical IATF(Artificial Insemination in fixed time). The experiment was developed with 68 cows, submitted to the following protocol: D0 at 08:00 h, and received an intravaginal P4 release device associated with intramuscular (IM) Estradiol Benzoate. D8 at 08:00 h, the P4 device was withdrawn and Dinoprost tromethamine (IM) was administered; Estradiol Cypionate (IM) and Equine Chorionic Gonadotrophin (IM). D10 at 08:00 h, 34 cows were inseminated in the uterine body and 34 inseminated intracornual, ipsilateral to the ovary with the preovulatory follicle. The diagnosis of gestation was performed 45 days after AI by transrectal ultrasonography. The conception rate found with semen deposition in the uterine body was 32%, while deep intracornual deposition was 26%. The results demonstrated a non-significant difference between the techniques (p> 0.05). Thus, uncontractual fixed-time artificial insemination does not improve the conception rate when compared to artificial insemination at fixed transcervical times. However, more studies need to be done to standardize the technique.