SENSITIVITY OF THE BACTERIA AGAINST THE LOW CONCENTRATION OF PESTICIDES AND HEAVY METALS

Abstract

– For this study ten frequently used pesticides were used Viz; Cypermethrin, Chloropyriphos, Acephate, Spinosad, Captan, Carbendazim, Metalaxyl, Mancozeb, Chlorothalonil, 2-4-D as per European Union standard concentration. Seven heavy metals were used viz; Copper (cu), Lead (Pb), Cadmium (Cd), Chromium (Cr), Nickel (Ni), Arsenic (As), Mercury (Hg) as per Indian standards. Total 21 isolates were isolated and screened for cypermethrin, 16 for chloropyriphos, 11 for acephate, 16 for spinosad, captan 22, carbendazim 16, metalaxyl 12, mancozeb 9, chlorothalonil 14 and for 2-4-D 20 at low concentration of these pesticides. Among the heavy metals total 30 isolates were isolated and screened for copper, for lead 14, cadmium 10, chromium 20, nickel 10, and for arsenic 12. These isolates were tested for sensitivity against low concentration of pesticides and heavy metals. The result of experiment showed that very few isolates CAR-8, CTG-10, DIM-5 showed sensitivity to carbendazim at 2.5 ppm, captan at 1ppm and 2-4-D at 2.5 ppm respectively and isolates NIV-7 and COM-2 showed sensitivity to heavy metals like nickel at 0.04ppm and copper at 0.01ppm concentration.