ASSESSING THE BIOACTIVE CONSTITUENTS OF DODONEA VISOSA (VIRAALI) THROUGH GC-MS

Abstract

–The present effort was intended to investigate Dodonea visosa for phytochemical compounds and characterize the chemical constituents of plant using GC-MS. The shade dried leaf, stem, internode, callus parts of plant powder of DodoneaVisosa was extracted with Methanol overnight, filtered and concentrated. The GC Clarus 500 (Perkin Elmer) used in the investigation employed a column packed with Elite-5MS (5%Diphenyl / 95% Dimethyl poly siloxane, 30mm x 0.25mm x0.25µmdf) and the components were separated using Helium (1ml/min) as the carrier gas. The 2 µl sample extract injected into the instrument was detected by the Turbo mass gold detector (Perkin Elmer) with the aid of the Turbomass 5.2 software. The GC-MS analysis provided different peaks determining the presence of seven phytochemicals for Viraali Leaf dry powder, two Phyto-chemicals for Viraali Stem dry powder, six Phytochemicals for Viraali Internode dry powder, three phytochemicals for Viraali Callus dry powder. The phytochemical and GC-MS profiling methanolic extract of Dodonea visosa revealed the presence of bioactive compounds with important medicinal properties. Hence, the presence of these phytochemicals could be responsible for the therapeutic effects of the plant.