Evaluated some Efflux-Pump gene Expression under ciprofloxacin stress in Pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa is an opportunistic bacteria; adaptation in different environments makes them very aggressive and life-threatening pathogens for humans, especially patients who suffer from weak immunity. This study aimed to evaluate the gene expression of each mexA and mexB gene in Pseudomonas aeruginosa isolates, which were isolated from clinical samples; screening of efflux pumps in multidrug P. aeruginosa phenotypically was done by the Cather wheel method. Study genes expression of efflux pump genes under ciprofloxacin antibiotic stress and Et. Br were done in different concentrations of cip and EtBr. One hundred and ten (110) isolates of Pseudomonas spp. were collected from different hospitals in Baghdad city, (50) isolates of Pseudomonas aeruginosa identified and confirmed were done. The results appeared according to the type of clinical sources the fiftyisolate distributed as 35 (70 %) burns swab samples, 10 (20 %) from wounds swabs, and 5 (10 %) from ear discharge. The results of mexA Ct values ranged from 32.1 to 36.79 with an average of 34.337, and the results of mexB Ct values ranged from 14.74 to 19.96 with an average of 16.791 compared to the rpsL (Ct = 11.14) reference gene. The results showed that the mean of Ct of gene expression was noticed when put under stress of Et.Br. + CIP and CIP in mexA were 37.195 and 34.702, respectively, compared to control 34.337; on the other hand, the gene expression between both treatments was over-expressed in the treatment of CIP 32 compared to CIP+Et.Br. The Cather wheel method expression results were noticed when stressed, Et.Br. + CIP and CIP in mexB gene expression were 19.626 and 18.872, respectively, compared to control 16.791. In conclusion, compared to the control, both treatments did not induce the expression of mexB genes; on the other hand, the gene expression between both treatments was expressed in the treatment of CIP 32 compared to CIP+Et.Br. Keywords: Pseudomonas aeruginosa, mexA, mexB, Cather Wheel.