Intestinal morphometric changes associated with the use of non-antibiotic feed additives in broiler chicks challenged with Salmonella Enteritidis

IF 2.7 3区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Journal of Animal Science and Technology Pub Date : 2023-10-16 DOI:10.5187/jast.2023.e113

Laelia Felix, Alexandre Moreira Filho, Maylane Rayane Santos, Mauro Saraiva, Oliveiro Freitas Neto, Patrícia Givisiez, Celso Oliveira

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引用次数: 0

Abstract

Non-antibiotic feed additives stand as a potential alternative for antimicrobial growth promoters, but their effects in the gastrointestinal tract of broiler chicks suffering early infection are poorly understood. This study aimed to investigate the effects of two non-antibiotic feed additives (a postbiotic and a sanguinarine-based phytobiotic) on the gut morphology and body weight gain of broiler chicks challenged with Salmonella enterica serovar Enteritidis (SE). Birds (n=144) were distributed according to a 2 × 3 factorial in a completely randomized design with the following treatments: non-challenged chicks fed control diet (SHAM-DCO), postbiotic (SHAM-PFC), or sanguinarine-based compound (SHAM-SAN) and SE-challenged chicks fed control diet (SE-DCO), postbiotic (SE-PFC), and sanguinarine-based compound (SE-SAN). Birds from each treatment were euthanized at 3-, 7-, and 14-days post inoculation and samples were collected for SE counting and intestinal morphometry. Weight gain was determined at 14 days post-inoculation. Lower (p ≤ 0.05) Salmonella counts were observed in birds fed diets containing PFC at 3- and 7-days post inoculation. SE-challenged chicks showed greater crypt depth (p ≤ 0.05) and lamina propria thickness (p ≤ 0.05) and smaller villus:crypt ratio (p ≤ 0.05) at the different sampling periods. Overall, birds fed PFC or SAN showed decreased lamina propria thickness (p ≤ 0.05), greater villus height (p ≤ 0.05), villus:crypt ratio (p ≤ 0.05), and larger villus area (p ≤ 0.05) compared with those fed the control diet (DCO). SAN supplementation improved body weight (p ≤ 0.05) and weight gain (p ≤ 0.05) until 14 days post-hatch compared with the control diet. Both feed additives (PFC and SAN) improved birds’ response to post-hatch Salmonella Enteritidis infection, evidenced by beneficial changes in gut morphology. These effects highlight the potential of these feed additives to improve gut health of broiler chicks during the initial rearing phase.

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肠炎沙门氏菌攻毒肉鸡肠道形态变化与使用非抗生素饲料添加剂相关

非抗生素饲料添加剂是抗菌生长促进剂的潜在替代品，但其对早期感染肉鸡胃肠道的影响尚不清楚。本研究旨在研究两种非抗生素饲料添加剂(后生物制剂和以血碱为基础的植物制剂)对感染肠沙门氏菌/血清型肠炎(SE)的肉仔鸡肠道形态和增重的影响。采用完全随机设计，按2 × 3因子分布144只鸡，分别饲喂对照饲粮(SHAM-DCO)、益生菌后(SHAM-PFC)或以血碱为基础的复合物(SHAM-SAN)和对照饲粮(SE-DCO)、益生菌后(SE-PFC)和以血碱为基础的复合物(SE-SAN)。各组分别于接种后3、7、14天实施安乐死，采集标本进行SE计数和肠道形态测定。接种后14天测定体重增加。低(& lt; italic>术中;/ italic>≤0.05)<italic>沙门氏菌</italic>在接种后3天和7天分别观察含PFC饲料的雏鸟的计数。硒挑战雏鸡的隐窝深度更大(<italic>p</italic>≤0.05)和固有层厚度(<italic>p</italic>≤0.05)和更小的绒毛:隐窝比(<italic>p</italic>≤0.05)。总体而言，喂食PFC或SAN的鸟类显示固有层厚度减少(<italic>p</italic>≤0.05)，绒毛高度较大(<italic>p</italic>≤0.05)，绒毛:隐窝比(<italic>p</italic>≤0.05)，绒毛面积较大(<italic>p</italic>≤0.05)，与对照组(DCO)相比有显著差异。补充SAN可改善体重(<italic>p</italic>≤0.05)和体重增加(<italic>p</italic>≤0.05)，直至孵化后14 d。两种饲料添加剂(PFC和SAN)都改善了鸟类对孵化后沙门氏菌的反应。肠炎感染，由肠道形态的有益改变证明。这些影响突出了这些饲料添加剂在饲养初期改善肉鸡肠道健康的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Animal Science and Technology Agricultural and Biological Sciences-Food Science

CiteScore

4.50

自引率

8.70%

发文量

审稿时长

7 weeks

期刊介绍： Journal of Animal Science and Technology (J. Anim. Sci. Technol. or JAST) is a peer-reviewed, open access journal publishing original research, review articles and notes in all fields of animal science. Topics covered by the journal include: genetics and breeding, physiology, nutrition of monogastric animals, nutrition of ruminants, animal products (milk, meat, eggs and their by-products) and their processing, grasslands and roughages, livestock environment, animal biotechnology, animal behavior and welfare. Articles generally report research involving beef cattle, dairy cattle, pigs, companion animals, goats, horses, and sheep. However, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will also be considered for publication. The Journal of Animal Science and Technology (J. Anim. Technol. or JAST) has been the official journal of The Korean Society of Animal Science and Technology (KSAST) since 2000, formerly known as The Korean Journal of Animal Sciences (launched in 1956).