<i>Corrigendum to</i>: Cadmium thiosulfate complexes can be assimilated by a green alga via a sulfate transporter but do not increase Cd toxicity

IF 2 4区 环境科学与生态学 Q3 CHEMISTRY, ANALYTICAL
Frédéric Boily, Claude Fortin, Peter G. C. Campbell
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引用次数: 0

Abstract

Environmental context Thiosulfate is present in natural waters, especially those influenced by sulfide oxidation, and it has a marked affinity for metals such as cadmium. Normally the binding of cadmium by thiosulfate would be expected to reduce the metal&#x2019;s bioavailability. However, here we demonstrate that algal uptake of cadmium is enhanced in the presence of thiosulfate, indicating that Cd can enter the alga via a novel route as an intact Cd-thiosulfate complex. Rationale For a given free metal ion activity in the exposure solution, the Biotic Ligand Model assumes that metal uptake will be independent of the various ligands present in solution that are buffering [Mz+]. In this context, we have evaluated cadmium bioavailability in the absence or presence of thiosulfate, using Chlamydomonas reinhardtii as the test alga. Methodology Short-term exposures (&#x2264;41&#x2009;min) were run with a fixed concentration of the free Cd2+ ion (3.0&#x2009;&#xb1;&#x2009;0.1&#x2009;nM), buffered with either nitrilotriacetate or thiosulfate, to determine Cd uptake. Subsequent long-term exposures (72&#x2009;h) over a range of free Cd2+ concentrations were used to determine the effects of Cd on algal growth. Results Contrary to Biotic Ligand Model predictions, Cd uptake was enhanced when Cd2+ was buffered with thiosulfate. Removal of sulfate from this exposure medium increased Cd uptake; conversely, if [SO42&#x2212;] was increased, Cd uptake decreased. In the absence of thiosulfate, Cd uptake was unaffected by changes in [SO42&#x2212;]. In the long-term exposures, the cellular Cd quota needed to reduce algal growth by 50% was significantly higher in the presence of thiosulfate than in its absence. Discussion In the presence of thiosulfate, Cd can enter the algal cell not only by cation transport but also by transport of the intact Cd-thiosulfate complex via the anion transporter responsible for sulfate uptake. We speculate that some of the Cd taken up by anion transport remains in complexed form and is less bioavailable than the Cd that enters the cell via cation transport.
<i>更正</i>:硫代硫酸镉配合物可以通过硫酸盐转运体被绿藻吸收,但不会增加镉毒性
& lt; sec>硫代硫酸盐存在于自然水体中,特别是那些受硫化物氧化影响的水体,它对镉等金属具有明显的亲和力。通常情况下,硫代硫酸盐与镉的结合会降低金属的生物利用度。然而,在这里,我们证明了硫代硫酸盐的存在增强了藻类对镉的吸收,这表明Cd可以作为完整的Cd-硫代硫酸盐复合物通过一种新的途径进入藻类。& lt; / sec>& lt; sec>基本原理对于暴露溶液中给定的自由金属离子活度,生物配体模型假设金属摄取将独立于溶液中存在的各种缓冲配体[M<sup><i>z</i>+</sup>]。在此背景下,我们利用莱茵衣藻(Chlamydomonas reinhardtii</i>)评估了在不存在或存在硫代硫酸盐的情况下镉的生物利用度。作为测试藻类。& lt; / sec>& lt; sec>短期暴露(≤41 min)与固定浓度的游离Cd<sup>2+</sup>离子(3.0 ± 0.1 nM),用三乙酸硝基或硫代硫酸盐缓冲,以测定镉的吸收。随后的长期暴露(72 h)在自由Cd<sup>2+</sup>浓度测定了镉对藻类生长的影响。& lt; / sec>& lt; sec>结果与生物配体模型预测相反,Cd<sup>2+</sup>用硫代硫酸盐缓冲。从暴露介质中去除硫酸盐增加了镉的吸收;相反,如果[SO<sub>4</sub><sup>2−</sup>]增加,则Cd吸收减少。在没有硫代硫酸盐的情况下,Cd的吸收不受[SO<sub>4</sub><sup>2−</sup>]变化的影响。在长期暴露中,在硫代硫酸盐存在时,使藻类生长减少50%所需的细胞Cd配额明显高于不存在硫代硫酸盐的情况。& lt; / sec>& lt; sec>在硫代硫酸盐存在的情况下,Cd不仅可以通过阳离子运输进入藻类细胞,还可以通过负责硫酸盐摄取的阴离子转运体运输完整的Cd-硫代硫酸盐复合物。我们推测阴离子运输所吸收的一些镉仍以络合形式存在,其生物可利用性低于通过阳离子运输进入细胞的镉。& lt; / sec>
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来源期刊
Environmental Chemistry
Environmental Chemistry 环境科学-分析化学
CiteScore
4.50
自引率
0.00%
发文量
0
审稿时长
2.7 months
期刊介绍: Environmental Chemistry publishes manuscripts addressing the chemistry of the environment (air, water, earth, and biota), including the behaviour and impacts of contaminants and other anthropogenic disturbances. The scope encompasses atmospheric chemistry, geochemistry and biogeochemistry, climate change, marine and freshwater chemistry, polar chemistry, fire chemistry, soil and sediment chemistry, and chemical aspects of ecotoxicology. Papers that take an interdisciplinary approach, while advancing our understanding of the linkages between chemistry and physical or biological processes, are particularly encouraged. While focusing on the publication of important original research and timely reviews, the journal also publishes essays and opinion pieces on issues of importance to environmental scientists, such as policy and funding. Papers should be written in a style that is accessible to those outside the field, as the readership will include - in addition to chemists - biologists, toxicologists, soil scientists, and workers from government and industrial institutions. All manuscripts are rigorously peer-reviewed and professionally copy-edited. Environmental Chemistry is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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