ZmDRR206 functions in maintaining cell wall integrity during maize seedling growth and defense response to external stresses

IF 6 1区 农林科学 Q1 AGRONOMY
Tao Zhong , Suining Deng , Mang Zhu , Xingming Fan , Mingliang Xu , Jianrong Ye
{"title":"ZmDRR206 functions in maintaining cell wall integrity during maize seedling growth and defense response to external stresses","authors":"Tao Zhong ,&nbsp;Suining Deng ,&nbsp;Mang Zhu ,&nbsp;Xingming Fan ,&nbsp;Mingliang Xu ,&nbsp;Jianrong Ye","doi":"10.1016/j.cj.2023.09.007","DOIUrl":null,"url":null,"abstract":"<div><p>Plants adaptively change their cell wall composition and structure during their growth, development, and interactions with environmental stresses. Dirigent proteins (DIRs) contribute to environmental adaptations by dynamically reorganizing the cell wall and/or by generating defense compounds. A maize DIR, ZmDRR206, was previously reported to play a dominant role in regulation of storage nutrient accumulation in endosperm during maize kernel development. Here we show that ZmDRR206 mediates maize seedling growth and disease resistance by coordinately regulating biosynthesis of cell wall components for cell-wall integrity (CWI) maintenance. Expression of <em>ZmDRR206</em> was induced in maize seedlings upon pathogen infection. <em>ZmDRR206</em> overexpression in maize resulted in reduced seedling growth and photosynthetic activity but increased disease resistance and drought tolerance, revealing a tradeoff between growth and defense. Consistently, <em>ZmDRR206</em> overexpression reduced the contents of primary metabolites and down-regulated genes involved in photosynthesis, while increasing the contents of major cell wall components, defense phytohormones, and defense metabolites, and up-regulated genes involved in defense and cell-wall biosynthesis in seedlings. <em>ZmDRR206-</em>overexpressing seedlings were resistant to cell-wall stress imposed by isoxaben, and ZmDRR206 physically interacted with ZmCesA10, which is a cellulose synthase unit. Our findings suggest a mechanism by which ZmDRR206 coordinately regulates biosynthesis of cell-wall components for CWI maintenance during maize seedling growth, and might be exploited for breeding strong disease resistance in maize.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001356/pdfft?md5=4327b3ba9fefeb6dfb35b37318eaed14&pid=1-s2.0-S2214514123001356-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123001356","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

Abstract

Plants adaptively change their cell wall composition and structure during their growth, development, and interactions with environmental stresses. Dirigent proteins (DIRs) contribute to environmental adaptations by dynamically reorganizing the cell wall and/or by generating defense compounds. A maize DIR, ZmDRR206, was previously reported to play a dominant role in regulation of storage nutrient accumulation in endosperm during maize kernel development. Here we show that ZmDRR206 mediates maize seedling growth and disease resistance by coordinately regulating biosynthesis of cell wall components for cell-wall integrity (CWI) maintenance. Expression of ZmDRR206 was induced in maize seedlings upon pathogen infection. ZmDRR206 overexpression in maize resulted in reduced seedling growth and photosynthetic activity but increased disease resistance and drought tolerance, revealing a tradeoff between growth and defense. Consistently, ZmDRR206 overexpression reduced the contents of primary metabolites and down-regulated genes involved in photosynthesis, while increasing the contents of major cell wall components, defense phytohormones, and defense metabolites, and up-regulated genes involved in defense and cell-wall biosynthesis in seedlings. ZmDRR206-overexpressing seedlings were resistant to cell-wall stress imposed by isoxaben, and ZmDRR206 physically interacted with ZmCesA10, which is a cellulose synthase unit. Our findings suggest a mechanism by which ZmDRR206 coordinately regulates biosynthesis of cell-wall components for CWI maintenance during maize seedling growth, and might be exploited for breeding strong disease resistance in maize.

ZmDRR206 在玉米幼苗生长过程中维持细胞壁完整性以及对外部胁迫的防御响应中发挥作用
植物在生长、发育以及与环境压力相互作用的过程中会适应性地改变细胞壁的组成和结构。定向蛋白(DIR)通过动态重组细胞壁和/或生成防御化合物来促进环境适应。之前有报道称,玉米 DIR ZmDRR206 在玉米籽粒发育过程中对胚乳中贮藏养分的积累起着主导调控作用。在这里,我们发现 ZmDRR206 通过协调调节细胞壁成分的生物合成来维持细胞壁完整性(CWI),从而介导玉米幼苗的生长和抗病性。病原体感染后,ZmDRR206 在玉米幼苗中被诱导表达。ZmDRR206 在玉米中的过表达导致幼苗生长和光合作用活性降低,但抗病性和耐旱性增强,揭示了生长和防御之间的权衡。一致的是,ZmDRR206 的过表达降低了幼苗初级代谢产物的含量,下调了参与光合作用的基因,而增加了主要细胞壁成分、防御植物激素和防御代谢产物的含量,上调了参与防御和细胞壁生物合成的基因。ZmDRR206过表达的幼苗能抵抗异噁苯施加的细胞壁胁迫,ZmDRR206与纤维素合成酶单元ZmCesA10有物理相互作用。我们的研究结果表明,在玉米幼苗生长过程中,ZmDRR206协调调控细胞壁成分的生物合成以维持CWI的机制,可用于培育玉米的强抗病性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信