MALDI-TOF MS and MICRONAUT susceptibility test as recent bacteriological applications of Klebsiella spp isolated from large animals in Egypt.

Abstract

Keywords The purpose of this study is to assess the accuracy of the MALDI TOF technique in identifying Klebsiella spp. Plus, application of MICRONAUT susceptibility testing for detection of MDR klebsiella isolates. A total of 293 samples (233 nasal swabs, 45 milk sample, 10 anal swabs and 5 vaginal swabs ) were collected from 258 clinically diseased animals. All samples were examined and all typical colonies were analyzed biochemically and confirmed by MALDI TOF method. All confirmed klebislla spp. isolates were tested for antimicrobial susceptibility to 18 antimicrobials. Typical colonies were confirmed biochemically in 30 samples as klebsiella (10.2%). They were identified as klebsiella spp. (n=24), k. pneumoniae (n=3) and enterobacter spp. (n=3) by MALDI TOF. Antibiotic susceptibility testing of 27 k. pneumoniea strains by using MICRONAUT susceptibility testing discovered that all of the isolated strains were sensitive to 7/16 tested antibiotics (43.75%) amoxicillin/clavulanic acid, ceftiofur, cephalothin, enrofloxacin, gentamicin, spectinomycin, trimethoprim/sulfamethoxazol, complete resistance was observed to only 6 antibiotics, i.e., ampicillin erythromycin , tiamulin, tilmicosin, penicillin G, tulathromycin. All strains showed intermediate susceptible to florfenicol except only one strain. Eighty five percent of the isolates recorded sensitivity to colistin and the remaining show intermediate susceptibility. Ten strains were unsusceptible to tetracycline (6 resistance, 4 intermediate) and the other were susceptible to tetracycline.