Prevalence of Bacillus cereus in milk and some milk products in Egypt

Ahmed Elgushi, Hend Elbarbary, Hamdi Mohammed, Dalia Elmasry
{"title":"Prevalence of Bacillus cereus in milk and some milk products in Egypt","authors":"Ahmed Elgushi, Hend Elbarbary, Hamdi Mohammed, Dalia Elmasry","doi":"10.21608/bvmj.2023.228010.1699","DOIUrl":null,"url":null,"abstract":"Keywords Bacillus cereus is a spore-forming bacterium that causes food spoilage and poisoning. This study aimed to evaluate the prevalence of enterotoxigenic Bacillus cereus in 180 dairy samples across three cites (Tanta, El-Santa, and El-Mahalla El-Kubra) in El-Gharbia Governorate, Egypt, including raw milk, milk powder and Ras cheese ( 60 of each). Samples were collected from December 2022 to April 2023 and analyzed using traditional isolation, biochemical identification with VITEK 2, and PCR targeting the nhe A gene as a virulence factor for Bacillus cereus . Traditional isolation on selective media indicated a prevalence of 1.66%, 24.99% and 4.99% in raw milk, milk powder and Ras cheese, respectively. However, VITEK 2 only confirmed that Bacillus cereus in milk powder isolates was 24.99%. PCR detection of the nhe A gene revealed that Bacillus cereus in the examined samples completely matched with traditional isolation. Overall, 10.5% of samples were positive for enterotoxigenic Bacillus cereus by PCR. Compared to PCR, traditional isolation showed 100% sensitivity and specificity, while VITEK 2 had 79% sensitivity and 100% specificity. Although biochemical methods like VITEK 2 enabled rapid automated identification, PCR detection of virulence genes provided superior sensitivity for identifying enterotoxigenic Bacillus cereus of clinical significance from dairy samples. Traditional culture techniques remain essential for isolating target bacteria prior to PCR. This study provided insights into the prevalence of enterotoxigenic Bacillus cereus contamination in some Egyptian dairy products.","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Benha Veterinary Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/bvmj.2023.228010.1699","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Keywords Bacillus cereus is a spore-forming bacterium that causes food spoilage and poisoning. This study aimed to evaluate the prevalence of enterotoxigenic Bacillus cereus in 180 dairy samples across three cites (Tanta, El-Santa, and El-Mahalla El-Kubra) in El-Gharbia Governorate, Egypt, including raw milk, milk powder and Ras cheese ( 60 of each). Samples were collected from December 2022 to April 2023 and analyzed using traditional isolation, biochemical identification with VITEK 2, and PCR targeting the nhe A gene as a virulence factor for Bacillus cereus . Traditional isolation on selective media indicated a prevalence of 1.66%, 24.99% and 4.99% in raw milk, milk powder and Ras cheese, respectively. However, VITEK 2 only confirmed that Bacillus cereus in milk powder isolates was 24.99%. PCR detection of the nhe A gene revealed that Bacillus cereus in the examined samples completely matched with traditional isolation. Overall, 10.5% of samples were positive for enterotoxigenic Bacillus cereus by PCR. Compared to PCR, traditional isolation showed 100% sensitivity and specificity, while VITEK 2 had 79% sensitivity and 100% specificity. Although biochemical methods like VITEK 2 enabled rapid automated identification, PCR detection of virulence genes provided superior sensitivity for identifying enterotoxigenic Bacillus cereus of clinical significance from dairy samples. Traditional culture techniques remain essential for isolating target bacteria prior to PCR. This study provided insights into the prevalence of enterotoxigenic Bacillus cereus contamination in some Egyptian dairy products.
蜡样芽孢杆菌在埃及牛奶及部分奶制品中的流行情况
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信