The BnTFL1–BnGF14nu–BnFD module regulates flower development and plant architecture in Brassica napus

IF 6 1区 农林科学 Q1 AGRONOMY
Jianjun Wang , Chi Zhang , Youpeng Chen , Yanan Shao , Meifang Liao , Qian Hou , Weitang Zhang , Yang Zhu , Yuan Guo , Zijin Liu , Christian Jung , Mingxun Chen
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引用次数: 0

Abstract

Flower development and plant architecture determine the efficiency of mechanized harvest and seed yield in Brassica napus. Although TERMINAL FLOWER 1 (AtTFL1) is a regulator of flower development in Arabidopsis thaliana, the function and regulatory mechanism of TFL1 orthologs in B. napus remains unclear. Six BnTFL1 paralogs in the genome of the B. napus inbred line ‘K407’ showed steadily increasing expression during vernalization. CRISPR/Cas-induced mutagenesis of up to four BnTFL1 paralogs resulted in early flowering and alteration of plant architecture, whereas seed yield was not altered in BnTFL1 single, double, or triple mutants. Six BnTFL1 paralogs, but not BnaA02.TFL1, showed an additive and conserved effect on regulating flowering time, total and terminal flower number, and plant architecture. BnaA10.TFL1 regulates flower development by interacting with BnaA08.FD through the protein BnaA05.GF14nu, resulting in the transcriptional repression of floral integrator and floral meristem identity genes. These findings about the regulatory network controlling flower development and plant architecture present a promising route to modifying these traits in B. napus.

BnTFL1-BnGF14nu-BnFD 模块调控甘蓝型油菜的花发育和植株结构
花的发育和植株结构决定了油菜机械化收获的效率和种子产量。尽管终端花1(AtTFL1)是拟南芥花发育的调控因子,但TFL1直向同源物在油菜中的功能和调控机制仍不清楚。油菜近交系'K407'基因组中的6个BnTFL1旁系物在春化过程中的表达量稳步上升。CRISPR/Cas诱导诱变多达四个BnTFL1旁系亲本会导致早花和植株结构的改变,而BnTFL1单突变体、双突变体或三突变体的种子产量不会改变。六个 BnTFL1 旁系亲本(但不包括 BnaA02.TFL1)在调节开花时间、总花和顶花数量以及植株结构方面表现出相加的保守效应。BnaA10.TFL1通过蛋白BnaA05.GF14nu与BnaA08.FD相互作用,导致花整合基因和花分生组织特征基因的转录抑制,从而调控花的发育。这些关于控制花发育和植物结构的调控网络的发现为改变油菜的这些性状提供了一条很有前景的途径。
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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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