Genetic features of the Puumala virus (Hantaviridae: Orthohantavirus) identified in the Moscow region

Q3 Medicine

Voprosy virusologii Pub Date : 2023-09-21 DOI:10.36233/0507-4088-177

Ekaterina A. Blinova, Marat T. Makenov, Evgeny S. Morozkin, Ivan S. Kholodilov, Marina V. Fedorova, Olga B. Zhurenkova, German V. Roev, Kamil F. Khafizov, Ludmila S. Karan

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Abstract

Introduction. Puumala virus (family Hantaviridae, genus Orthohantavirus) is distributed in most regions of the European part of Russia. However, information about its genetic variants circulating on the territory of the Central Federal District is extremely scarce. Materials and methods. Rodents tissue samples were tested after reverse transcription by PCR for the presence of hantaviral RNA. The amplified fragments of the L segment were sequenced by the Sanger method. For two samples, sequences of all three segments were obtained using the NGS method. Phylogenetic trees were built in the MEGA-X software. Results. Puumala virus was found in six samples. Based on the phylogenetic analysis of sequences of three segments, the obtained genetic variants belong to the sublineage previously designated as W-RUS. Conclusion. A genetic variant of the Puumala virus, belonging to the subline W-RUS, circulates on the territory of the Volokolamsk district of Moscow region.

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在莫斯科地区发现的普玛拉病毒(汉坦病毒科:正汉坦病毒)的遗传特征

介绍。普玛拉病毒(汉坦病毒科，正汉坦病毒属)分布在俄罗斯欧洲部分的大部分地区。然而，在中央联邦区境内流传的有关其遗传变异的信息极为稀少。材料和方法。用PCR反转录检测鼠组织样本中汉坦病毒RNA的存在。扩增后的L片段用Sanger法测序。对于两个样本，使用NGS方法获得了所有三个片段的序列。在MEGA-X软件中构建系统发育树。结果。在6个样本中发现了普玛拉病毒。根据三个片段序列的系统发育分析，获得的遗传变异属于先前指定的W-RUS亚系。结论。普玛拉病毒的一种遗传变异，属于W-RUS亚型，在莫斯科州沃洛科拉姆斯克地区传播。

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来源期刊

Voprosy virusologii Medicine-Infectious Diseases

CiteScore

2.00

自引率

0.00%

发文量

期刊介绍： The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.

Genetic features of the Puumala virus (Hantaviridae: <i>Orthohantavirus</i>) identified in the Moscow region

Abstract