Molecular identification of Methicillin-resistant staphylococcus aureus (MRSA) and Vancomycin- resistant staphylococcus aureus (VRSA) strains isolated from milk and milk products.

Pansee Zahid, Enas Soliman, Zeinab Mahdy
{"title":"Molecular identification of Methicillin-resistant staphylococcus aureus (MRSA) and Vancomycin- resistant staphylococcus aureus (VRSA) strains isolated from milk and milk products.","authors":"Pansee Zahid, Enas Soliman, Zeinab Mahdy","doi":"10.21608/bvmj.2023.222598.1680","DOIUrl":null,"url":null,"abstract":"Keywords In developing countries, resistance to antimicrobial agents is a serious public health problem, and the indiscriminate use of antimicrobials is a key contributor to the development of resistant bacterial diseases. The following research was aimed to determine the in-vitro antimicrobial activity of (23 strains) of Staphylococcus aureus isolated from 80 random samples of milk and dairy products (soft cheese “Kareish and white” and Roomy cheese) 20 samples from each product, with molecular identification of their resistant genes. The results declared that all isolated strains were totally resistant to Ampicillin (100%), Amoxicillin, and Erythromycin (86.9%), then Oxacillin (65.2%), while all other strains showed intermediate susceptibility to Vancomycin, Cefoxitin, Cefotaxime, and Norfloxacin. While Gentamicin had the highest level of susceptibility (78.3%). Moreover, by the cultivation of the isolated strains on oxacillin resistance screening agar base plates “ORSAB,” there were 15/23 MRSA strains; also, 5/23 strains showed resistance to Vancomycin and grow well in vancomycin screening agar. The molecular identification for the mecA gene and vanA gene was made on seven strains (3 strains resistant to both Oxacillin and Vancomycin, two strains with intermediate resistance, and two strains sensitive to them) isolated from different products (milk, Kariesh cheese, soft white cheese, and Roomy cheese). The results showed that all the samples carried the mecA gene, while only two samples had the vanA gene (isolated from milk). Staphylococcus aureus Antimicrobial resistant mecA gene vanA gene","PeriodicalId":8803,"journal":{"name":"Benha Veterinary Medical Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Benha Veterinary Medical Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21608/bvmj.2023.222598.1680","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Keywords In developing countries, resistance to antimicrobial agents is a serious public health problem, and the indiscriminate use of antimicrobials is a key contributor to the development of resistant bacterial diseases. The following research was aimed to determine the in-vitro antimicrobial activity of (23 strains) of Staphylococcus aureus isolated from 80 random samples of milk and dairy products (soft cheese “Kareish and white” and Roomy cheese) 20 samples from each product, with molecular identification of their resistant genes. The results declared that all isolated strains were totally resistant to Ampicillin (100%), Amoxicillin, and Erythromycin (86.9%), then Oxacillin (65.2%), while all other strains showed intermediate susceptibility to Vancomycin, Cefoxitin, Cefotaxime, and Norfloxacin. While Gentamicin had the highest level of susceptibility (78.3%). Moreover, by the cultivation of the isolated strains on oxacillin resistance screening agar base plates “ORSAB,” there were 15/23 MRSA strains; also, 5/23 strains showed resistance to Vancomycin and grow well in vancomycin screening agar. The molecular identification for the mecA gene and vanA gene was made on seven strains (3 strains resistant to both Oxacillin and Vancomycin, two strains with intermediate resistance, and two strains sensitive to them) isolated from different products (milk, Kariesh cheese, soft white cheese, and Roomy cheese). The results showed that all the samples carried the mecA gene, while only two samples had the vanA gene (isolated from milk). Staphylococcus aureus Antimicrobial resistant mecA gene vanA gene
牛奶及乳制品中耐甲氧西林金黄色葡萄球菌(MRSA)和耐万古霉素金黄色葡萄球菌(VRSA)的分子鉴定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信