A lead conversion method for staining thiamine pyrophosphatase and other phosphatases in rat brain after thin layer polyacrylamide gel isoelectric focusing.

Basic and applied histochemistry Pub Date : 1988-01-01
P Mazzarello, M Poloni, C Patrini, D Bosone, G Rindi
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引用次数: 0

Abstract

Rat brain thiamine pyrophosphatase (TPPase) was separated by thin layer polyacrylamide gel isoelectric focusing (IF) and stained with a modification of the lead conversion method of Allen and Hyncik (1963). 10 bands with TPPase activity were observed in the pH range 4.6-7.1. The overall IF pattern of TPPase was similar to that of uridine diphosphatase and inosine diphosphatase but was clearly different from that of adenosine diphosphatase, p-nitrophenyl phosphatase, alpha-naphthyl phosphatase and thiamine monophosphatase. A semiquantitative assessment of TPPase isoenzymes has been performed using laser densitometry.

薄层聚丙烯酰胺凝胶等电聚焦后大鼠脑内硫胺素焦磷酸酶及其他磷酸酶的铅转换染色方法。
采用薄层聚丙烯酰胺凝胶等电聚焦法(IF)分离大鼠脑硫胺素焦磷酸酶(TPPase),并对Allen和Hyncik(1963)的铅转化法进行改进染色。在pH 4.6 ~ 7.1范围内观察到10条具有TPPase活性的条带。TPPase的总体IF模式与尿苷二磷酸酶和肌苷二磷酸酶相似,但与腺苷二磷酸酶、对硝基苯磷酸酶、α -萘磷酸酶和硫胺素单磷酸酶有明显差异。用激光密度测定法对TPPase同工酶进行了半定量评估。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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