Red Dragon Fruit Peel Extract (Hylocereus lemairei) as an Alternative Dye in Worm Egg Staining

Scientia Pub Date : 2023-10-30 DOI:10.52221/jurkes.v10i2.333
Ary Nurmalasari, Dinda Ayunastiti, Atun Farihatun, Rahayu Nirmatul M
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引用次数: 0

Abstract

Helminthiasis is still a problem in Indonesia, and worm infections often occur without symptoms, so the disease receives less attention. Identification can be done by several methods, one of which is by direct examination using 2% eosin dye. However, 2% eosin dye is included in synthetic dyes that can harm ul to health. The alternative used is to utilize natural ingredients. Red dragon fruit Hylocereus lemairei is a natural material that contains anthocyanin substances where in an acidic atmosphere anthocyanin substances provide a red colour so that it can be used as an alternative dye to replace eosin. The aim of this study was to determine the microscopic picture of worm egg preparations using alternative dyes of red dragon fruit peel extract. The research method used is an experimental method. The results showed that the red dragon fruit peel extract dye with a concentration variation of 2%, 5%, and 10% did not colour the worm eggs. So the red dragon fruit peel extract dye is still not optimal for use as an alternative dye for coloring worm eggs.
红龙果皮提取物在虫卵染色中的应用研究
蠕虫病在印度尼西亚仍然是一个问题,蠕虫感染通常没有症状,因此这种疾病受到的关注较少。鉴别方法有几种,其中一种是用2%的伊红染料直接检测。然而,合成染料中含有2%的伊红染料,对人体健康有害。另一种选择是使用天然成分。红龙果是一种含有花青素物质的天然材料,在酸性环境中,花青素物质会呈现红色,因此它可以作为替代伊红的染料。研究了红龙果皮提取物替代染料制备虫卵的显微图像。所采用的研究方法是实验方法。结果表明,2%、5%和10%浓度变化的红龙果皮浸膏染料对虫卵无显色作用。因此,红龙果皮提取物染料仍然不是用于虫卵染色的最佳替代染料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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