The aim of this study was to estimate random match frequency of randomly acquired characteristics (RAC-RMF) for laboratory-simulated crime scene impressions. Part I of this investigation reports this metric using a dataset of more than 160 questioned impressions created in blood and deposited on tile. A total of 759 RACs were identified in the blood impressions and compared to RACs with positional similarity in test impressions from 1,299 unrelated outsoles. Geometric similarity was quantified using a combination of visual comparisons and mathematical modeling based on percent area overlap. Results indicated that RACs in blood impressions were typically smaller, and therefore exhibited a two-thirds increase in the number of indistinguishable pairs compared to their mated test impressions. For shoes contributing at least one RAC, relative RAC-RMF values 0.0008 were encountered at a rate between 3.4% and 34% for the blood impressions examined in this study. Part II of this investigation provides analogous results based on dust impressions deposited on paper and tile. Although the results in Part I and Part II are specific to randomly acquired characteristics and do not translate into an impression-wide RMF estimate, this research shows that RACs in questioned impressions of the type expected in casework co-occur in position and geometry with RACs in non-mated test impressions. Since theoretical models have traditionally been the basis for estimating RAC-RMF in footwear, the overall contribution of this research to the forensic footwear community is a calibration of this estimate based on empirical data.