Immunoreactivity of infiltrating macrophages during gastric ulcer healing in rats

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES

Turkish Journal of Veterinary & Animal Sciences Pub Date : 2023-10-17 DOI:10.55730/1300-0128.4318

ERKMEN TUĞRUL EPİKMEN, EMRAH İPEK, RECAİ TUNCA

{"title":"Immunoreactivity of infiltrating macrophages during gastric ulcer healing in rats","authors":"ERKMEN TUĞRUL EPİKMEN, EMRAH İPEK, RECAİ TUNCA","doi":"10.55730/1300-0128.4318","DOIUrl":null,"url":null,"abstract":"This study investigated a timeline of changes in the immunoreactivity of infiltrating macrophages during gastric ulcer healing. To this end, gastric tissue samples were obtained from an acetic acid-induced gastric ulcer model in rats on the 1st, 3rd, 7th, and 14th days of ulcer induction. Ulcerated gastric tissues were subjected to histologic and immunohistochemical evaluations for macrophage subtypes. The number of Iba-1+ macrophages in the gastric ulcer area significantly increased on day 3, reaching a maximum number on day 7, followed by a decrease on day 14. No Gal-3+ macrophages were seen in the gastric ulcer area until day 14. Interestingly, CD68 reacted with macrophages, (myo)fibroblast-like spindle-shaped cells, and endothelial cells in the gastric ulcer area. There was a significant increase in the α-SMA+ myofibroblasts and desmin+ microvessels on days 7 and 14. The increase in the number of Iba-1+ macrophages was followed by the appearance of α-SMA+ myofibroblasts and desmin+ blood vessels. These results suggest that (i) different macrophage subtypes are involved in gastric ulcer healing, (ii) Iba-1+ macrophages, observed in the early stages of gastric healing, participate in proinflammatory and regenerative activities, (iii) Gal-3+ macrophages, seen in the late stages of healing, contribute to proinflammatory response and tissue repair, and (iv) CD68 is not a macrophage-specific marker.","PeriodicalId":23357,"journal":{"name":"Turkish Journal of Veterinary & Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.5000,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Veterinary & Animal Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.55730/1300-0128.4318","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

This study investigated a timeline of changes in the immunoreactivity of infiltrating macrophages during gastric ulcer healing. To this end, gastric tissue samples were obtained from an acetic acid-induced gastric ulcer model in rats on the 1st, 3rd, 7th, and 14th days of ulcer induction. Ulcerated gastric tissues were subjected to histologic and immunohistochemical evaluations for macrophage subtypes. The number of Iba-1+ macrophages in the gastric ulcer area significantly increased on day 3, reaching a maximum number on day 7, followed by a decrease on day 14. No Gal-3+ macrophages were seen in the gastric ulcer area until day 14. Interestingly, CD68 reacted with macrophages, (myo)fibroblast-like spindle-shaped cells, and endothelial cells in the gastric ulcer area. There was a significant increase in the α-SMA+ myofibroblasts and desmin+ microvessels on days 7 and 14. The increase in the number of Iba-1+ macrophages was followed by the appearance of α-SMA+ myofibroblasts and desmin+ blood vessels. These results suggest that (i) different macrophage subtypes are involved in gastric ulcer healing, (ii) Iba-1+ macrophages, observed in the early stages of gastric healing, participate in proinflammatory and regenerative activities, (iii) Gal-3+ macrophages, seen in the late stages of healing, contribute to proinflammatory response and tissue repair, and (iv) CD68 is not a macrophage-specific marker.

查看原文本刊更多论文

大鼠胃溃疡愈合过程中浸润性巨噬细胞的免疫反应性

本研究探讨了浸润性巨噬细胞在胃溃疡愈合过程中免疫反应性变化的时间轴。为此，在溃疡诱导的第1、第3、第7、第14天，取乙酸诱导大鼠胃溃疡模型胃组织标本。对溃烂的胃组织进行巨噬细胞亚型的组织学和免疫组织化学评估。胃溃疡区Iba-1+巨噬细胞数量在第3天显著增加，第7天达到最大值，第14天开始减少。直到第14天，胃溃疡区域未见Gal-3+巨噬细胞。有趣的是，CD68与胃溃疡区域的巨噬细胞、(肌)成纤维细胞样纺锤形细胞和内皮细胞发生反应。第7天和第14天α-SMA+肌成纤维细胞和desmin+微血管明显增加。Iba-1+巨噬细胞数量增加后，出现α-SMA+肌成纤维细胞和desmin+血管。这些结果表明:(1)不同的巨噬细胞亚型参与胃溃疡愈合;(2)在胃愈合早期观察到的Iba-1+巨噬细胞参与促炎和再生活动;(3)在愈合晚期观察到的Gal-3+巨噬细胞参与促炎反应和组织修复;(4)CD68不是巨噬细胞特异性标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Turkish Journal of Veterinary & Animal Sciences 农林科学-兽医学

CiteScore

1.30

自引率

0.00%

发文量

审稿时长

24 months

期刊介绍： The Turkish Journal of Veterinary and Animal Sciences is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK). Accepts English-language manuscripts on all aspects of veterinary medicine and animal sciences. Contribution is open to researchers of all nationalities. Original research articles, review articles, short communications, case reports, and letters to the editor are welcome. Manuscripts related to economically important large and small farm animals, poultry, equine species, aquatic species, and bees, as well as companion animals such as dogs, cats, and cage birds, are particularly welcome. Contributions related to laboratory animals are only accepted for publication with the understanding that the subject is crucial for veterinary medicine and animal science. Manuscripts written on the subjects of basic sciences and clinical sciences related to veterinary medicine, nutrition, and nutritional diseases, as well as the breeding and husbandry of the above-mentioned animals and the hygiene and technology of food of animal origin, have priority for publication in the journal. A manuscript suggesting that animals have been subjected to adverse, stressful, or harsh conditions or treatment will not be processed for publication unless it has been approved by an institutional animal care committee or the equivalent thereof. The editor and the peer reviewers reserve the right to reject papers on ethical grounds when, in their opinion, the severity of experimental procedures to which animals are subjected is not justified by the scientific value or originality of the information being sought by the author(s).