Atherosclerosis preventive effects of marrubiin against (TNF-α)-induced oxidative stress and apoptosis

IF 1.2 Q3 CARDIAC & CARDIOVASCULAR SYSTEMS
Ailar Nakhlband, Alireza Garjani, Nazli Saeedi, Yadollah Omidi, Samad Ghaffari, Jaleh Barar, Morteza Eskandani
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Abstract

Introduction: Atherosclerosis is a complicated cascade of inflammatory processes, oxidative stress, and apoptosis, making it the most prevalent cardiovascular disease. The onset and progression of cardiovascular diseases are greatly influenced by oxidative stress. Targeting oxidative stress is an effective strategy for treating such diseases. Marrubiin is a bioactive furan labdane diterpenoid acts as a strong antioxidant to protect against oxidative damage. This study aimed to investigate the protective effects of marrubiin against oxidative stress and apoptosis in a cellular model of the vascular system. Methods: Human umbilical vein endothelial cells were treated with varying concentration of marrubiin and its IC50 value was determined. The antioxidant potential of marrubiin was assessed by measuring the intracellular level of glutathione (GSH) using a colorimetric technique. Since apoptosis plays a significant role in the plaque rupture, the study also evaluated the protective effects of marrubiin on the expression of key genes involved in apoptotic pathways. Results: Cells treated with marrubiin showed increased GSH levels compared to cell therapy control cells, indicating marrubiin’s ability to counteract the effects of TNF-α’s on GSH levels. Furthermore real-time PCR analysis demonstrated that marrubiin upregulated Bcl-xl while downregulating caspase3 and Nox4 in treated cells. These findings suggest that marrubiin protects against apoptosis and oxidative stress. Conclusion: Based on our findings, marrubiin is recommended as a preventive/therapeutic treatment for diseases caused by elevated intracellular reactive oxygen species levels in cardiovascular diseases.
马鲁比宾对(TNF-α)诱导的氧化应激和细胞凋亡的动脉粥样硬化预防作用
动脉粥样硬化是一个复杂的级联炎症过程,氧化应激和细胞凋亡,使其成为最常见的心血管疾病。氧化应激对心血管疾病的发生和发展有很大的影响。靶向氧化应激是治疗此类疾病的有效策略。Marrubiin是一种具有生物活性的呋喃二萜,是一种强抗氧化剂,可以防止氧化损伤。本研究旨在探讨马鲁比醇对血管系统细胞模型氧化应激和细胞凋亡的保护作用。方法:用不同浓度的马鲁比醇处理人脐静脉内皮细胞,测定其IC50值。采用比色法测定细胞内谷胱甘肽(GSH)水平,评价马芦比星的抗氧化潜能。由于细胞凋亡在斑块破裂中起着重要作用,本研究还评估了马鲁比宾对凋亡通路相关关键基因表达的保护作用。结果:与细胞治疗对照细胞相比,马鲁比星处理的细胞显示GSH水平升高,表明马鲁比星能够抵消TNF-α对GSH水平的影响。此外,实时PCR分析表明,马鲁比因在处理细胞中上调Bcl-xl,下调caspase3和Nox4。这些研究结果表明,马鲁比林可以防止细胞凋亡和氧化应激。结论:基于我们的研究结果,马鲁比宾被推荐作为心血管疾病中由细胞内活性氧水平升高引起的疾病的预防/治疗药物。
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来源期刊
Journal of Cardiovascular and Thoracic Research
Journal of Cardiovascular and Thoracic Research CARDIAC & CARDIOVASCULAR SYSTEMS-
CiteScore
2.00
自引率
0.00%
发文量
22
审稿时长
7 weeks
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