Border disease virus and chlamydophila abortus co-infection in aborted sheep foetuses

Pub Date : 2023-10-18 DOI:10.12681/jhvms.30570

M Şevik

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Abstract

Abortion in sheep and goats is one of the most important reproductive problems that affect small ruminants breeding. Non-infectious factors and infectious agents such as bacterial, viral, mycotic and parasitic infectious agents can cause abortion in sheep and goats. A high rate of abortion (43.75%) was observed in two sheep flocks in Niğde Province in Turkey during the lambing season in 2016. To determine the reason of abortion, aborted sheep foetuses (n = 3) and EDTA whole blood samples (n = 3) from mother of the foetuses were collected from two sheep flocks. Aborted foetuses and buffy coat cells of the EDTA whole blood samples were used for total nucleic acid extraction. Extracted nucleic acids were analysed for akabane virus (AKAV), bluetongue virus (BTV), peste des petits ruminants virus (PPRV), pestiviruses, Schmallenberg virus (SBV), Brucella spp., Chlamydophila abortus (C. abortus), Coxiella burnetii and Listeria monocytogenes. Border disease virus (BDV) RNA and C. abortus DNA were detected in three aborted sheep foetuses whereas other investigated infectious agents were not detected. Additionally, EDTA whole blood samples from mother of the foetuses were also found BDV positive. To the best of my knowledge, this is the first report on BDV and C. abortus co-infection in aborted sheep foetuses.

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绵羊流产胎儿边境病病毒与流产衣原体共感染

绵羊和山羊的流产是影响小型反刍动物繁殖的最重要的生殖问题之一。非传染性因素和感染因子，如细菌、病毒、真菌和寄生虫感染因子可导致绵羊和山羊流产。2016年产羔季，土耳其Niğde省2个羊群的流产率较高(43.75%)。为了确定流产的原因，我们从两个羊群中采集了流产羊胎儿(n = 3)和胎儿母亲的EDTA全血样本(n = 3)。用流产胎儿和EDTA全血标本的灰白色被细胞提取总核酸。对提取的阿卡巴纳病毒(AKAV)、蓝舌病病毒(BTV)、小反刍兽疫病毒(PPRV)、鼠疫病毒、施马伦伯格病毒(SBV)、布鲁氏菌、流产衣原体(C. abortus)、伯纳克希菌和单核增生李斯特菌进行核酸检测。在3个流产羊胎中检测到边界病病毒RNA和流产C. DNA，而未检测到其他被调查的传染因子。此外，胎儿母亲的EDTA全血样本也发现BDV阳性。据我所知，这是第一次报道BDV和C. abortus在流产羊胎儿中同时感染。

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