IDENTIFICATION OF ‘TONGKOL’ FISH IN KEDONGANAN FISH MARKET, KUTA, BADUNG DISTRICT, BALI

Abstract

‘Tongkol’ fishes are species in the family Scombridae. There are many types of ‘tongkol’ fishes, namely longtail tuna, eastern little tuna, frigate tuna and bullet tuna. Morphologically, ‘tongkol’ species are similar to one another. This can result in errors in recording the production of ‘tongkol’ species. The purpose of this study was to identify ‘tongkol’ species collected from Kedonganan Market, Badung, Bali using the COI (Cytochrome C Oxidase Subunit I) gene. Two species of ‘tongkol’ fish were purchased from Kedonganan Market, Badung, Bali. DNA isolation was carried out from fish fins using chelex, followed by amplification of the COI gene segment. The PCR products were then electrophoresed and sequenced. DNA sequences were matched to the database using the Basic Local Alignment Search Tool (BLAST) found in NCBI. Extraction yielded DNA with a concentration of 5.91 ng/ml with a ratio of A260/A280 = 1.3 for sample 1, while for sample 2 the concentration of DNA was 6.27 ng/ml with a ratio of A260/A280 = 1.33. The resulting PCR product is about 700bp in size. The results of the COI gene sequences had a gene size length of 682 bp for both fish species. BLAST analysis yielded a percent identity of 99.84-100% with eastern little tuna (Euthynnus affinis) and bullet tuna (Auxis rochei).