Detection and quantification of antiviral drug tenofovir using silver nanoparticles and surface enhanced Raman spectroscopy (SERS) with spatially resolved hotspot selection

IF 4.1 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY
Marguerite R. Butler, Jana Hrncirova, Terry A. Jacot, Sucharita Dutta, Meredith R. Clark, Gustavo F. Doncel, John B. Cooper
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引用次数: 0

Abstract

This study introduces a convenient and ultra-sensitive method of detection and quantification of the antiviral drug, tenofovir (TFV), by surface-enhanced Raman spectroscopy (SERS). Novel spatially resolved instrumentation for spectral acquisition and subsequent statistical analysis for hot spot selection was developed for convenient quantification of TFV in an aqueous matrix. Methods of statistical analysis include the use of partial least squares (PLS) regression vector analysis and spectral ranking by quality indices computed using CHAOS theory. Hydroxylamine-reduced Ag colloidal nanoparticles evaporated to dryness on an aluminum well-plate were used as the SERS substrate. To our knowledge, quantification of TFV down to 25 ng/mL by SERS, comprising clinically relevant concentrations, has not been previously reported. Furthermore, in this work we propose a novel method of quantification of aqueous TFV standards by SERS using statistical treatment of data by PLS and CHAOS theory. Based on these data, we propose future studies to develop a method of TFV detection and quantification in biological samples, beneficial to clinicians for rapid assessment of drug adherence during the treatment and prevention of viral diseases.
利用纳米银粒子和具有空间分辨热点选择的表面增强拉曼光谱(SERS)检测和定量抗病毒药物替诺福韦
本研究介绍了一种方便、超灵敏的抗病毒药物替诺福韦(TFV)的表面增强拉曼光谱(SERS)检测和定量方法。开发了用于光谱采集和后续热点选择统计分析的新型空间分辨仪器,以方便定量水相基质中的TFV。统计分析方法包括利用偏最小二乘(PLS)回归向量分析和利用混沌理论计算质量指标的光谱排序。在铝孔板上蒸发至干燥的羟胺还原银胶体纳米粒子作为SERS底物。据我们所知,通过SERS将TFV量化至25 ng/mL,包括临床相关浓度,此前尚未报道。此外,在这项工作中,我们提出了一种新的方法,通过使用PLS和混沌理论对数据进行统计处理的SERS定量含水TFV标准。基于这些数据,我们建议未来研究开发一种生物样品中TFV检测和定量方法,有助于临床医生在病毒性疾病的治疗和预防过程中快速评估药物依从性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Frontiers in Nanotechnology
Frontiers in Nanotechnology Engineering-Electrical and Electronic Engineering
CiteScore
7.10
自引率
0.00%
发文量
96
审稿时长
13 weeks
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