Dynamics of acid phosphatase production by cell suspension system and its further characterization

IF 0.7 4区农林科学 Q3 AGRONOMY

Emirates Journal of Food and Agriculture Pub Date : 2023-08-21 DOI:10.9755/ejfa.2023.3141

Saima Khan, Sharada Mallubhotla, Malik Muzafar Manzoor, Sandeep Singh Pagoch, Ashok Ahuja

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引用次数: 0

Abstract

The current study compares the influence of media engineered media on production of acid phophatase (APase) with its characterization in suspension cell cultures of Atropa acuminata, authenticated by rbcL and ITS. Various parameters like pH, inoculum size, temperature, nutrient sources in the medium were optimized for APase hyperproduction in in vitro conditions. APase production was accomplished to be evidently amplified at 5% inoculum size (94.90 U/gFW), pH 5.0(99.75 U/gFW) and incubation temperature of 30°C (91.5U/gFW). Sucrose as carbon and ammonium nitrate as nitrogen sources were eminently suited for maximum enzyme harvest (91.20 U/gFW and 94.56 U/gFW). The APase activity was observed to be distinctly increased in phosphate (Pi) starved suspension cells (197.84 U/gFW). Overall, 6.5 fold increase in APase activity was achieved as compared to the activity before optimization studies. Hence the present Apase production and its activity from Atropa acuminata suspension culture may have apparent use for future industrial, agricultural and biotechnological application. Keywords: Acuminata; Acid phosphatase; p-nitrophenylphosphate; Suspension cells; Pi-starvation

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细胞悬浮体系生产酸性磷酸酶的动力学及其进一步表征

本研究通过rbcL和its验证，比较了培养基工程培养基对酸性磷酸酶(APase)产生的影响及其在阿特拉帕(Atropa acuminata)悬浮细胞培养中的特性。在体外条件下，对pH、接种量、温度、培养基营养来源等参数进行了优化。在接种量为5% (94.90 U/gFW)、pH为5.0(99.75 U/gFW)、培养温度为30℃(91.5U/gFW)的条件下，APase产量明显增加。蔗糖作为碳源和硝酸铵作为氮源最适合酶收获(91.20 U/gFW和94.56 U/gFW)。磷酸(Pi)饥饿悬浮细胞(197.84 U/gFW)的APase活性明显升高。总的来说，与优化研究之前相比，APase活性提高了6.5倍。因此，从悬培养中提取的Apase及其活性在未来的工业、农业和生物技术方面具有明显的应用价值。关键词:Acuminata;酸性磷酸酶;p-nitrophenylphosphate;悬浮细胞;Pi-starvation

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来源期刊

Emirates Journal of Food and Agriculture AGRONOMYFOOD SCIENCE & TECHNOLOGY&nb-FOOD SCIENCE & TECHNOLOGY

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The "Emirates Journal of Food and Agriculture [EJFA]" is a unique, peer-reviewed Journal of Food and Agriculture publishing basic and applied research articles in the field of agricultural and food sciences by the College of Food and Agriculture, United Arab Emirates University, United Arab Emirates.