Molecular detection of blaTEM, blaSHV,and blaCTX-M genes among Uropathogenic Escherichia coli isolated from cases with urinary tract infection in Erbil city-Iraq

Kharman K Ahmed ,, Aumed A Hawezy
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 Objective: evaluate how frequently blaTEM, blaSHV, and blaCTX-M genes were detected in E. coli isolated from UTIs.
 Patients and Methods: We collected 54 midstream urine samples from patients with symptomatic UTIs, in all age groups, from the outpatient department in Erbil hospitals from October 1, 2021 to April 1, 2022 for the isolation of E. coli. All samples were analyzed for the detection of blaTEM, blaSHV, and blaCTX genes using the polymerase chain reaction (PCR) method.
 Results: Most of the samples were taken from females (61.11%); according to their ages, they were divided into two groups, and most of the samples (74.07%) were taken from patients below 40 years old. PCR testing for all ESBL-producing E. coli isolate samples revealed that 16S rRNA 797 was the most frequently detected gene in all analyzed samples (100%), while it was less frequently detected in blaCTX 585 (48.15%).
 Conclusion: Colonization with S. aureus and MRSA inversely correlated with younger This found that elevated ESBL genes in E. coli isolated from symptomatic UTIs in our community increase the risk of possible resistance.","PeriodicalId":11202,"journal":{"name":"Diyala Journal of Medicine","volume":"13 87","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diyala Journal of Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26505/djm.v25i1.1021","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract

Background: Urinary tract infections (UTIs) are the most popular type of diagnosed bacterial illness, and the most frequent cause of bacteria responsible for UTIs is Escherichia coli (E. coli). β-lactamases are the most frequent resistance for gram-negative bacteria to beta-lactam antibiotics, especially in E. coli. The number of patients infected by extended-spectrum β-lactamases (ESBLs) producing E. coli was rising and regarded as a significant global health problem. Objective: evaluate how frequently blaTEM, blaSHV, and blaCTX-M genes were detected in E. coli isolated from UTIs. Patients and Methods: We collected 54 midstream urine samples from patients with symptomatic UTIs, in all age groups, from the outpatient department in Erbil hospitals from October 1, 2021 to April 1, 2022 for the isolation of E. coli. All samples were analyzed for the detection of blaTEM, blaSHV, and blaCTX genes using the polymerase chain reaction (PCR) method. Results: Most of the samples were taken from females (61.11%); according to their ages, they were divided into two groups, and most of the samples (74.07%) were taken from patients below 40 years old. PCR testing for all ESBL-producing E. coli isolate samples revealed that 16S rRNA 797 was the most frequently detected gene in all analyzed samples (100%), while it was less frequently detected in blaCTX 585 (48.15%). Conclusion: Colonization with S. aureus and MRSA inversely correlated with younger This found that elevated ESBL genes in E. coli isolated from symptomatic UTIs in our community increase the risk of possible resistance.
伊拉克埃尔比勒市尿路感染尿路致病性大肠埃希菌blaTEM、blaSHV和blaCTX-M基因的分子检测
背景:尿路感染是最常见的细菌性疾病,而导致尿路感染的最常见细菌是大肠杆菌。β-内酰胺酶是革兰氏阴性菌对β-内酰胺类抗生素最常见的耐药性,尤其是在大肠杆菌中。产生大肠杆菌的广谱β-内酰胺酶(ESBLs)感染的患者数量正在上升,并被视为一个重大的全球健康问题。目的:评价blaTEM、blaSHV和blaCTX-M基因在UTIs分离的大肠杆菌中的检出率。患者和方法:从2021年10月1日至2022年4月1日在埃尔比勒医院门诊部收集了54例有症状的尿路感染患者的中游尿液样本,用于分离大肠杆菌。采用聚合酶链反应(PCR)方法对所有样本进行blblatem、blaSHV和blaCTX基因的检测。 结果:以女性标本居多(61.11%);按年龄分为两组,40岁以下患者占样本的绝大部分(74.07%)。对所有产esbl的大肠杆菌分离样本进行PCR检测,结果显示16S rRNA 797是所有分析样本中检出频率最高的基因(100%),而在blaCTX 585中检出频率较低(48.15%)。结论:金黄色葡萄球菌和MRSA的定植与年龄呈负相关。本研究发现,我们社区症状性尿路感染分离的大肠杆菌中ESBL基因升高,增加了可能的耐药风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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